S100A12 蛋白, Human

The S100A12 protein is a calcium, zinc, and copper binder that regulates inflammation and immune responses. As a DAMP molecule, it activates innate immune cells through AGER, triggering pro-inflammatory pathways. S100A12 Protein, Human is the recombinant human-derived S100A12 protein, expressed by E. coli , with tag free. The total length of S100A12 Protein, Human is 92 a.a., with molecular weight of ~11.0 kDa.

S100A12 是一种钙、锌和铜结合蛋白，在调节炎症过程和免疫反应中发挥着关键作用。其促炎功能包括招募白细胞、促进细胞因子和趋化因子的产生以及调节白细胞粘附和迁移。S100A12 作为警报素或危险相关分子模式 (DAMP) 分子，通过与晚期糖基化终末产物 (AGER) 受体结合来激活先天免疫细胞。这种结合会触发 MAP 激酶和 NF-κ-B 等信号通路，从而产生促炎细胞因子以及 ICAM1 和 VCAM1 等细胞粘附分子的上调。作为化学引诱剂，它将单核细胞和肥大细胞吸引到炎症部位，诱导肥大细胞脱粒和激活。S100A12 还通过螯合 Zn(2+) 活性位点对基质金属蛋白酶（MMP2、MMP3 和 MMP9）产生抑制作用。此外，它还具有抗丝虫和抑制丝虫的活性，以及针对白色念珠菌的抗真菌特性以及针对大肠杆菌和铜绿假单胞菌的抗菌作用。S100A12 在钙、锌和铜离子存在下形成同二聚体和同寡聚体（四聚体或六聚体），并以钙依赖性方式与 AGER 和 CACYBP 相互作用。

Human

E. coli

Tag Free

P80511 (M1-E92)

6283  [NCBI]

MTKLEEHLEGIVNIFHQYSVRKGHFDTLSKGELKQLLTKELANTIKNIKDKAVIDEIFQGLDANQDEQVDFQEFISLVAIALKAAHYHTHKE

Approximately 11.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.