S100A13 蛋白, Mouse (N-His)

S100A13 Protein, acting as a homodimer, facilitates the export of signal peptide-lacking proteins through an alternative pathway, binding two calcium ions and one copper ion per subunit. It is crucial for the copper-dependent stress-induced export of IL1A and FGF1, with the calcium-free form binding to phosphatidylserine-containing lipid vesicles. S100A13 is part of a copper-dependent multiprotein complex, interacting with FGF1, SYT1, and IL1A. S100A13 Protein, Mouse (N-His) is the recombinant mouse-derived S100A13 protein, expressed by E. coli , with N-His labeled tag. The total length of S100A13 Protein, Mouse (N-His) is 98 a.a., with molecular weight of ~12 kDa.

S100A13 蛋白在缺乏信号肽并通过替代途径分泌的蛋白质的输出中发挥着至关重要的作用。它具有每个亚基结合两个钙离子和一个铜离子的能力，后者的结合不干扰钙结合。S100A13 对于铜依赖性应激诱导的 IL1A 和 FGF1 输出至关重要。有趣的是，无钙形式的蛋白质可以与含有磷脂酰丝氨酸的脂质囊泡结合，但不能与含有磷脂酰胆碱的脂质囊泡结合。S100A13 作为同二聚体发挥作用，与 FGF1 和 SYT1 一起属于铜依赖性多蛋白复合物的一部分。它还与 FGF1、SYT1 和 IL1A 相互作用。

Measured by its ability to enhance the outgrowth of SH-SY5Y cells. The ED₅₀ of this effect is 1.075 μg/mL, corresponding to a specific activity is 930.233 units/mg.

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  Measured by its ability to enhance the outgrowth of SH-SY5Y cells. The ED₅₀ of this effect is 1.075 μg/mL, corresponding to a specific activity is 930.233 units/mg.

Mouse

E. coli

N-His

P97352 (M1-K98)

20196

MAAETLTELEAAIETVVSTFFTFAGREGRKGSLNINEFKELATQQLPHLLKDVGSLDEKMKTLDVNQDSELRFSEYWRLIGELAKEVRKEKALGIRKK

Approximately 12 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.