S100B 蛋白, Human (His)

The S100B protein binds calcium and zinc ions, each with a different site on the monomer. Physiological levels of potassium ions disrupt the binding of calcium and zinc, affecting high-affinity sites. S100B Protein, Human (His) is the recombinant human-derived S100B protein, expressed by E. coli , with N-6*His labeled tag. The total length of S100B Protein, Human (His) is 92 a.a., with molecular weight of ~12.0 kDa.

S100B是一种蛋白质，具有结合钙离子和锌离子的作用。每个单体上的每个离子都有不同的结合位点。钾离子的生理浓度会干扰钙和锌的结合，特别是影响高亲和力的钙结合位点。S100B 作为神经营养因子发挥作用，促进星形细胞增多和轴突增殖。它还参与生热脂肪组织的神经支配，促进交感神经支配。S100B 可以通过释放激酶内的抑制性相互作用来激活 STK38 激酶。心肌梗死后，它与 AGER 相互作用，可能通过 ERK1/2 和 p53/TP53 信号传导促进心肌细胞凋亡。S100B 可能有助于 ATAD3A 的细胞质加工，防止聚集并促进线粒体定位。它还可以通过钙依赖性相互作用调节其他蛋白质的活性，例如含 TPR 的蛋白质。S100B 形成两条 α 链、两条 β 链或一条 α 和一条 β 链的二聚体。它与多种蛋白质相互作用，包括 CACYBP、ATAD3A、S100A6、CAPZA1、AGER、PPP5C、TPPP 和 CLSTN3 的亚型 CLSTN3beta，产生不同的功能后果。

Measured in a cell proliferation assay using THP-1 cells. The ED₅₀ for this effect is 0.09308 μg/mL, corresponding to a specific activity is 1.07×10⁴ units/mg.

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  Measured in a cell proliferation assay using THP-1 cells. The ED₅₀ for this effect is 0.09308 μg/mL, corresponding to a specific activity is 1.07×10⁴ units/mg.

Human

E. coli

N-6*His

P04271 (M1-E92)

6285  [NCBI]

MSELEKAMVALIDVFHQYSGREGDKHKLKKSELKELINNELSHFLEEIKEQEVVDKVMETLDNDGDGECDFQEFMAFVAMVTTACHEFFEHE

Approximately 12.0 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4 or PBS, 300 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.