SARS-CoV-2 Nucleocapsid 蛋白, Human (His)

The SARS-CoV-2 nucleocapsid protein plays a crucial role in assembling the virion, forming the helical ribonucleocapsid (RNP), and interacting with the viral genome and membrane protein M. In addition to its structural function, it also enhances subgenomic viral RNA transcription and overall replication. SARS-CoV-2 Nucleocapsid Protein, Human (His) is the recombinant human-derived SARS-CoV-2 Nucleocapsid protein, expressed by E. coli , with N-6*His labeled tag. The total length of SARS-CoV-2 Nucleocapsid Protein, Human (His) is 419 a.a., with molecular weight of ~48 kDa.

SARS-CoV-2 核衣壳蛋白在病毒粒子组装中发挥着关键作用，协调将正链病毒基因组 RNA 包装成螺旋核糖核衣壳 (RNP)，并与病毒基因组和膜蛋白 M 建立重要的相互作用。功能，该蛋白显着有助于亚基因组病毒 RNA 转录和整体病毒复制的效率。值得注意的是，它在调节宿主趋化因子功能方面表现出额外的作用，这种机制可能有利于病毒复制和传播。核衣壳蛋白通过分泌到细胞外空间来实现这种调节，在细胞外空间中它与宿主趋化因子竞争与宿主糖胺聚糖 (GAG) 的结合，从而可能破坏宿主防御机制并支持病毒生命周期。

1.Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-N at 2 μg/mL can bind SARS-CoV-2-N Antibody and the EC₅₀ is 0.9081 -8.795 ng/mL.
2.Immobilized SARS-CoV-2 Nucleocapsid protein, His Tag at 1 μg/mL (100 μL/well) can bind Anti-SARS-CoV- 2 Nucleocapsid Antibody. The ED₅₀ for this effect is 5.118 ng/mL.

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  Immobilized SARS-CoV-2 Nucleocapsid protein, His Tag at 1 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Nucleocapsid Antibody. The ED₅₀ for this effect is 5.118 ng/mL.

Human

E. coli

N-6*His

P0DTC9 (M1-A419)

43740575  [NCBI]

MSDNGPQNQRNAPRITFGGPSDSTGSNQNGERSGARSKQRRPQGLPNNTASWFTALTQHGKEDLKFPRGQGVPINTNSSPDDQIGYYRRATRRIRGGDGKMKDLSPRWYFYYLGTGPEAGLPYGANKDGIIWVATEGALNTPKDHIGTRNPANNAAIVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGDAALALLLLDRLNQLESKMSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKAYNVTQAFGRRGPEQTQGNFGDQELIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYTAAIKLDDKDPNFKDQVILLNKHIDAYKTFPPTEPKKDKKKKADETQALPQRQKKQQTVTLLPAADLDDFSKQLQQSMSSADSTQA

Approximately 48-50 kDa

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  Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm solution of 20 mM Tris-HC1, 0.5 M NaCl, 6% Trehalose, pH 8.0 or 50 mM Tris-HCL, 300 mM NaCl, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.