SARS-CoV-2 PLpro 蛋白

The multifunctional SARS-CoV-2 PP1ab protein is essential for viral RNA transcription and replication, utilizing proteases for multi-protein cleavage. It inhibits host translation by binding to the 40S subunit and blocks ribosomal mRNA entry channels, thereby hindering the antiviral response. SARS-CoV-2 PLpro Protein is the recombinant Virus-derived SARS-CoV-2 PLpro protein, expressed by E. coli , with tag free. The total length of SARS-CoV-2 PLpro Protein is 315 a.a., with molecular weight of ~34.0 kDa.

SARS-CoV-2 PP1ab 蛋白是一种对病毒 RNA 转录和复制至关重要的多功能成分，含有负责多蛋白裂解的蛋白酶。该蛋白通过与 40S 亚基的开放头构象结合，在抑制宿主翻译中发挥关键作用，其 C 末端阻碍核糖体 mRNA 进入通道，从而阻碍先天免疫或干扰素触发的抗病毒反应。nsp1-40S 核糖体复合物的形成导致宿主 mRNA 5'UTR 附近发生核酸内切裂解，从而促进其降解。值得注意的是，病毒 mRNA 由于其 5' 端前导序列，对 nsp1 介导的翻译抑制表现出更大的抵抗力。这种多方面的功能强调了 SARS-CoV-2 PP1ab 在操纵宿主细胞过程以促进病毒复制和逃避宿主防御方面的战略作用。

PLpro/papain-like protease(Plpro) can hydrolyze Arg-Leu-Arg-Gly-Gly-AMC to produce AMC, and the activity of PLpro is calculated by measuring the amount of product AMC produced at the excitation wavelength of 380nm and the emission wavelength of 460nm. The specific activity is 124.43pmol/min/μg.

Virus

E. coli

Tag Free

P0DTD1-1 (E1564-K1878)

43740578  [NCBI]

EVRTIKVFTTVDNINLHTQVVDMSMTYGQQFGPTYLDGADVTKIKPHNSHEGKTFYVLPNDDTLRVEAFEYYHTTDPSFLGRYMSALNHTKKWKYPQVNGLTSIKWADNNCYLATALLTLQQIELKFNPPALQDAYYRARAGEAANFCALILAYCNKTVGELGDVRETMSYLFQHANLDSCKRVLNVVCKTCGQQQTTLKGVEAVMYMGTLSYEQFKKGVQIPCTCGKQATKYLVQQESPFVMMSAPPAQYELKHGTFTCASEYTGNYQCGHYKHITSKETLYCIDGALLTKSSEYKGPITDVFYKENSYTTTIK

Approximately 34.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

溶液

Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 10 mM 2-Mercaptoethanol, 20% Glycerol, pH 7.5.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.