1. 重组蛋白
  2. Viral Proteins
  3. SARS-CoV-2 Proteins
  4. SARS-CoV-2 Spike Proteins
  5. SARS-CoV-2 S Protein
  6. SARS-CoV-2 S glycoprotein (D614G, HEK293, His)

SARS-CoV-2 S glycoprotein (D614G, HEK293, His)

目录号: HY-P72036
产品使用指南

SARS-CoV-2 S glycoprotein (D614G, HEK 293, His) 是一个带有 His 标签 的SARS-CoV-2 S 糖蛋白 D614G 蛋白。SARS-CoV-2 S glycoprotein (D614G) 是SARS-CoV-2 S 蛋白氨基酸 (aa) D614G 的变体,有更强的传染性。

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描述

SARS-CoV-2 S glycoprotein (D614G, HEK 293, His) is a SARS-CoV-2 S glycoprotein D614G protein with a His-flag. SARS-CoV-2 S glycoprotein (D614G) is a SARS-CoV-2 variant carrying the S protein amino acid (aa) change D614G, this variant has become the most prevalent form and has been associated with greater infectivity[1].

研究背景

SARS-CoV-2 导致 2019 年全球大流行冠状病毒病 (Covid-19)。SARS-CoV-2 属于冠状病毒家族。SARS-CoV-2 是本世纪已知的第三种引起肺炎并具有显着病死率的人类冠状病毒。
SARS-CoV-2 由四种结构蛋白组成:刺突蛋白(S 蛋白)、包膜蛋白( E)、膜蛋白 (M) 和核衣壳蛋白 (N)。
D614G 不会改变 S 蛋白的合成、加工或掺入 SARS-CoV-2 颗粒,但 D614G 对 ACE2 的亲和力会因以下原因而降低:更快的解离速度。D614G 取代导致多种细胞类型中的假病毒滴度显着升高,这表明刺突 G614 可能与增强进入细胞和增强气道复制有关[1][2]

种属

Virus

表达系统

HEK293

标签

C-10*His

蛋白编号

P0DTC2 (V16-R685, D614G)

基因 ID
蛋白结构
N-term
S glycoprotein (V16-R685, D614G)
Accession # P0DTC2
10*His
C-term
同用名
E2 Peplomer protein
氨基酸序列

VNLTTRTQLPPAYTNSFTRGVYYPDKVFRSSVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYFASTEKSNIIRGWIFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLGVYYHKNNKSWMESEFRVYSSANNCTFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPINLVRDLPQGFSALEPLVDLPIGINITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKYNENGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKKFLPFQQFGRDIADTTDAVRDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQGVNCTEVPVAIHADQLTPTWRVYSTGSNVFQTRAGCLIGAEHVNNSYECDIPIGAGICASYQTQTNSPRRAR

分子量

Approximately 77.8 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料
参考文献

SARS-CoV-2 S glycoprotein (D614G, HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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