1. 重组蛋白
  2. Viral Proteins
  3. SARS-CoV-2 Proteins
  4. SARS-CoV-2 Spike Proteins
  5. SARS-CoV-2 S Protein
  6. SARS-CoV-2 S glycoprotein (G476S, HEK293, His)

SARS-CoV-2 S glycoprotein (G476S, HEK293, His)

目录号: HY-P72041
产品使用指南

SSARS-CoV-2 S glycoprotein (G476S, HEK 293, His) 是带有 His 标签的 SARS-CoV-2 S glycoprotein G476S 蛋白。G476S 变体改变了ACE2 的结合亲和力。与武汉 SARS-CoV2 刺突蛋白相比,G476S 突变体对 ACE2 的亲和力降低。

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描述

SARS-CoV-2 S glycoprotein (G476S, HEK 293, His) is a SARS-CoV-2 S glycoprotein G476S protein with a His-flag. Variations at G476S alters the ACE2 binding affinity. G476S variants display reduced affinity to ACE2 in comparison to the Wuhan SARS-CoV2 spike protein[1].

研究背景

SARS-CoV-2 导致 2019 年全球大流行冠状病毒病 (Covid-19)。SARS-CoV-2 属于冠状病毒家族。SARS-CoV-2 是本世纪已知的第三种引起肺炎并具有显着病死率的人类冠状病毒。
SARS-CoV-2 由四种结构蛋白组成:刺突蛋白(S 蛋白)、包膜蛋白( E)、膜蛋白 (M) 和核衣壳蛋白 (N)。
D614G 不会改变 S 蛋白的合成、加工或掺入 SARS-CoV-2 颗粒,但 D614G 对 ACE2 的亲和力会因以下原因而降低:更快的解离速率[2][3]

种属

Virus

表达系统

HEK293

标签

C-10*His

蛋白编号

P0DTC2 (R319-F541, G476S)

基因 ID
蛋白结构
N-term
S glycoprotein (R319-F541, G476S)
Accession # P0DTC2
10*His
C-term
同用名
E2 Peplomer protein
氨基酸序列

RVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQASSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNF

分子量

Approximately 27.9 kDa

纯度

Greater than 85% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料
参考文献

SARS-CoV-2 S glycoprotein (G476S, HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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SARS-CoV-2 S glycoprotein (G476S, HEK293, His)
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HY-P72041
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