SARS-CoV-2 S glycoprotein (HEK293, His-mFc)

The SARS-CoV-2 S glycoprotein is critical in infection, binding to the ACE2 receptor and allowing viral particles to attach to the host cell membrane. Cleavage of S2/S2′ triggers cell membrane fusion or internalization via endocytosis. SARS-CoV-2 S glycoprotein (HEK293, His-mFc) is the recombinant Virus-derived SARS-CoV-2 S glycoprotein, expressed by HEK293 , with C-mFc, C-6*His labeled tag. The total length of SARS-CoV-2 S glycoprotein (HEK293, His-mFc) is 223 a.a., with molecular weight (glycosylation form) of ~66.0 kDa.

SARS-CoV-2 S 糖蛋白通过与主要受体宿主 ACE2 相互作用将病毒体附着到宿主细胞膜上，从而在感染中发挥至关重要的作用。S2/S2' 裂解后，与 ACE2 受体的结合引发细胞膜上的直接融合或病毒通过内吞作用内化，导致病毒体膜与宿主内体膜融合。此外，糖蛋白可能利用 NRP1/NRP2 和整合素作为替代进入受体，这可能解释了病毒在人类嗅觉上皮细胞中的趋向性。S 的柄域具有三个铰链，为头部提供了意想不到的方向自由度。作为 I 类病毒融合蛋白，该糖蛋白在病毒进入过程中，由宿主 TMPRSS2 或 CSTL 触发，经历广泛且不可逆的构象变化，导致病毒包膜与细胞质膜融合，并将病毒基因组 RNA 释放到宿主体内细胞的细胞质。该糖蛋白至少表现出三种构象状态：融合前天然、发夹前中间体和融合后发夹，其中卷曲螺旋区域在融合过程中采用发夹三聚体结构，促进病毒和病毒的并置和随后的融合。靶细胞膜。

Measured by its binding ability in a functional ELISA. Immobilized human ACE2 at 0.38147-50000 μg/mL can bind SARS-CoV-2-S1-RBD at 0.2 μg/well, the EC₅₀ of SARS-CoV-2-S1-RBD protein is 26.45-45.47 ng/mL.

Virus

HEK293

C-mFc;C-6*His

P0DTC2 (R319-F541)

43740568  [NCBI]

RVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNF

Approximately 66.0 kDa.The reducing (R) protein migrat es as 66 kDa in SDS-PAGE may be due to glycosylation.

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.