SARS-COV-2 S 蛋白 RBD (HEK293, His-Avi)

SARS-CoV-2 S1 Protein, in its Spike protein S1 form, initiates infection by attaching the virion to the cell membrane through host receptor interaction. Simultaneously, Spike protein S2' acts as a viral fusion peptide, revealing itself after S2 cleavage during virus endocytosis. SARS-COV-2 S Protein RBD (HEK293, His-Avi) is the recombinant Virus-derived SARS-COV-2 S protein RBD, expressed by HEK293 , with C-Avi, C-His labeled tag. The total length of SARS-COV-2 S Protein RBD (HEK293, His-Avi) is 214 a.a., with molecular weight of 32-42 kDa.

SARS-CoV-2 S1 蛋白在病毒感染的早期阶段发挥着至关重要的作用。刺突蛋白 S1 通过与宿主受体相互作用，促进病毒粒子附着在细胞膜上，从而启动感染过程。这种初始结合事件对于病毒随后进入宿主细胞至关重要。同时，刺突蛋白S2'作为病毒融合肽，在病毒内吞过程中S2裂解后发挥作用。S2'的暴露是病毒融合过程中的关键步骤，使病毒膜与内体膜融合，并促进病毒遗传物质释放到宿主细胞的细胞质中。这些 S1 和 S2' 功能的协同作用强调了 SARS-CoV-2 S1 蛋白在介导病毒进入和融合（病毒生命周期中的关键步骤）中的重要性。

Immobilized SARS-COV-2 Spike RBD, His Tag at 0.5 μg/mL (100μl/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC₅₀ of ≤9.4 ng/mL determined by ELISA.

Virus

HEK293

C-Avi;C-His

QHD43416.1 (R319-N532)

43740568  [NCBI]

RVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTN

32-42 kDa

Greater than 95% as determined by Tris-Bis PAGE.

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 8% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.