SARS-CoV-2 S 蛋白 RBD (HEK293)

The SARS-Cov-2 S glycoprotein is a SARS-Cov-2 S glycoprotein. The amino acid sequence 1261-1267a.a of the SARS-Cov-2 S glycoprotein is transmembrane. The SARS-Cov-2 S glycoprotein is a highly glycosylated trimer, each of which consists of 1260 amino acids (residues 14-1273), divided into four structural domains: the n-terminal domain (NTD), the c-terminal domain (CTD, also known as the receptor-binding domain, RBD), and two subdomains (SD1 and SD2). SARS-CoV-2 S Protein RBD (HEK293) is the recombinant Virus-derived SARS-CoV-2 S protein RBD, expressed by HEK293 , with tag free. The total length of SARS-CoV-2 S Protein RBD (HEK293) is 223 a.a., with molecular weight of approximately 34 kDa.

SARS-Cov-2 是一种包膜阳性单链RNA病毒，可导致2019冠状病毒病(COVID-19)。
SARS-CoV-2 具有 4 种结构蛋白，包括包膜蛋白 (E)、刺突或表面糖蛋白 (S)、膜蛋白(M) 和核衣壳蛋白 (N)。
SARS-Cov-2 S glycoprotein 位于病毒颗粒的外部，使冠状病毒具有冠状外观。
ARS-Cov-2 S glycoprotein 可以介导病毒颗粒附着并进入宿主细胞，是疫苗开发、抗体治疗和基于抗原的诊断测试的重要靶标^{[1][2][3][4][5]}。

Measured by its binding ability in a functional ELISA. Immobilized biotinylated SARS-CoV-2 S Protein RBD at 2 μg/mL (100 μL/well) can bind ACE2 Protein, Human (mFc) and the EC₅₀ is 20-80 ng/mL.

• 
  Immobilized Human ACE2 at 2 μg/mL (100 μL/well) can bind biotinylated SARS-CoV-2 S protein. The ED₅₀ for this effect is 10.95 ng/mL, corresponding to a specific activity is 9.13×10⁴ Unit/mg.

Virus

HEK293

Tag Free

YP_009724390.1 (R319-F541)

43740568  [NCBI]

RVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNF

approximately 34 kDa

• 
  Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 (Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.) or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.