1. 重组蛋白
  2. Viral Proteins Biotinylated Proteins
  3. SARS-CoV-2 Proteins
  4. SARS-CoV-2 Spike Proteins
  5. SARS-CoV-2 S1 Protein
  6. SARS-CoV-2 S1 蛋白 (Biotinylated, Omicron, B.1.1.529, HEK293, His-Avi)

SARS-CoV-2 S1 蛋白 (Biotinylated, Omicron, B.1.1.529, HEK293, His-Avi)

目录号: HY-P77635
产品使用指南

SARS-CoV-2 S1 蛋白以其刺突蛋白 S1 形式通过宿主受体相互作用将病毒体附着到细胞膜上来启动感染。同时,刺突蛋白 S2' 充当病毒融合肽,在病毒内吞过程中 S2 裂解后显露出来。SARS-CoV-2 S1 Protein (Biotinylated, Omicron, B.1.1.529, HEK293, His-Avi) 是重组的蛋白二聚体复合物,包含 SARS-CoV-2 S1 蛋白,由 HEK293 表达,带有 C-His, C-Avi 标签。SARS-CoV-2 S1 Protein (Biotinylated, Omicron, B.1.1.529, HEK293, His-Avi) ,分子量为 110-120 kDa。

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描述

SARS-CoV-2 S1 Protein, in its Spike protein S1 form, initiates infection by attaching the virion to the cell membrane through host receptor interaction. Simultaneously, Spike protein S2' acts as a viral fusion peptide, revealing itself after S2 cleavage during virus endocytosis. SARS-CoV-2 S1 Protein (Biotinylated, Omicron, B.1.1.529, HEK293, His-Avi) is a recombinant protein dimer complex containing Virus-derived SARS-CoV-2 S1 protein, expressed by HEK293 , with C-His, C-Avi labeled tag. SARS-CoV-2 S1 Protein (Biotinylated, Omicron, B.1.1.529, HEK293, His-Avi), has molecular weight of 110-120 kDa.

研究背景

SARS-CoV-2 S1 蛋白在病毒感染的早期阶段发挥着至关重要的作用。刺突蛋白 S1 通过与宿主受体相互作用,促进病毒粒子附着在细胞膜上,从而启动感染过程。这种初始结合事件对于病毒随后进入宿主细胞至关重要。同时,刺突蛋白S2'作为病毒融合肽,在病毒内吞过程中S2裂解后发挥作用。S2'的暴露是病毒融合过程中的关键步骤,使病毒膜与内体膜融合,并促进病毒遗传物质释放到宿主细胞的细胞质中。这些 S1 和 S2' 功能的协同作用强调了 SARS-CoV-2 S1 蛋白在介导病毒进入和融合(病毒生命周期中的关键步骤)中的重要性。

种属

Virus

表达系统

HEK293

标签

C-His;C-Avi

蛋白编号

QHO60594 (Q14-R683(A67V, HV69-70del, T95I, G142D, VYY143-145del, N211del, L212I, ins214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H))

基因 ID

/

蛋白结构
N-term
S1 Protein (Q14-R683 with multiple mutation)
Accession # QHO60594
His-Avi
C-term
同用名
S1 protein; Spike protein S1; Spike,S1 protein; S glycoprotein Subunit1
分子量

110-120 kDa

纯度

Greater than 95% as determined by Tris-Bis PAGE. Greater than 95% as determined by HPLC

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

SARS-CoV-2 S1 Protein (Biotinylated, Omicron, B.1.1.529, HEK293, His-Avi) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
SARS-CoV-2 S1 Protein (Biotinylated, Omicron, B.1.1.529, HEK293, His-Avi)
目录号:
HY-P77635
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