SARS-CoV S 蛋白 (HEK293, His-Myc)

The SARS-CoV S protein coordinates viral entry by interacting with human ACE2 and CLEC4M/DC-SIGNR receptors to attach viral particles to the cell membrane. It downregulates host tethering protein (BST2) through lysosomal degradation, antagonizing its antiviral activity. SARS-CoV S Protein (HEK293, His-Myc) is the recombinant Virus-derived SARS-CoV S protein, expressed by HEK293 , with N-10*His, C-Myc labeled tag. The total length of SARS-CoV S Protein (HEK293, His-Myc) is 222 a.a., with molecular weight of ~37 kDa.

SARS-CoV S 蛋白通过溶酶体降解下调宿主系链蛋白 (BST2)，从而抵消其抗病毒活性。在感染情况下，S 蛋白通过与宿主受体相互作用将病毒颗粒附着在细胞膜上，启动病毒进入过程。与人 ACE2 和 CLEC4M/DC-SIGNR 受体的结合，再加上病毒随后内化到宿主细胞的内体中，诱导 S 糖蛋白的构象变化。此外，组织蛋白酶 CTSL 的蛋白水解作用可能会揭示 S2 的融合肽，从而激活内涵体内的膜融合。这些精心策划的事件强调了 SARS-CoV S 蛋白在介导病毒进入和逃避宿主抗病毒防御方面的关键作用，揭示了其在 SARS-CoV 感染发病机制中的重要性。进一步的探索对于揭示这些过程背后的复杂分子机制并确定治疗干预的潜在目标至关重要。

Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV S-RBD at 2 μg/mL can bind Paguma larvata ACE2 , the EC₅₀ is 33.37-79.47 ng/mL.

Virus

HEK293

N-10*His;C-Myc

P59594 (R306-F527)

1489668  [NCBI]

RVVPSGDVVRFPNITNLCPFGEVFNATKFPSVYAWERKKISNCVADYSVLYNSTFFSTFKCYGVSATKLNDLCFSNVYADSFVVKGDDVRQIAPGQTGVIADYNYKLPDDFMGCVLAWNTRNIDATSTGNYNYKYRYLRHGKLRPFERDISNVPFSPDGKPCTPPALNCYWPLNDYGFYTTTGIGYQPYRVVVLSFELLNAPATVCGPKLSTDLIKNQCVNF

Approximately 37 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.