SBDS 蛋白, Mouse (His)

SBDS proteins are essential for the assembly of mature ribosomes and ribosome biogenesis. It cooperates with EFL1 to catalyze the GTP-dependent release of EIF6 from 60S preribosomes in the cytoplasm, activating the translation ability of ribosomes. SBDS Protein, Mouse (His) is the recombinant mouse-derived SBDS protein, expressed by E. coli , with N-His labeled tag. The total length of SBDS Protein, Mouse (His) is 250 a.a., with molecular weight of ~31 kDa.

SBDS是成熟核糖体组装和核糖体生物发生的复杂过程中不可或缺的因素。它与 EFL1 合作，协调 EIF6 从细胞质中的 60S 前核糖体中依赖 GTP 的释放，从而激活核糖体的翻译能力。该功能涉及促进 80S 核糖体的组装并确保 EIF6 再循环至细胞核，这对于 60S rRNA 加工和核输出至关重要。SBDS 对于维持蛋白质合成的正常水平至关重要，并可能有助于细胞应激抵抗、DNA 损伤反应和细胞增殖。该蛋白质与 60S 核糖体亚基形成关联，并与 NPM1、RPA1 和 PRKDC 等关键伙伴相互作用。它也是由 60S 核糖体亚基、SBDS 和 EFL1 组成的复合体的一部分。此外，SBDS 可能与 NIP7 和 CLN3 相互作用，强调了其在细胞过程中的多方面作用。

Mouse

E. coli

N-His

P70122 (M1-E250)

66711  [NCBI]

MSIFTPTNQIRLTNVAVVRMKRGGKRFEIACYKNKVVGWRSGVEKDLDEVLQTHSVFVNVSKGQVAKKEDLISAFGTDDQTEICKQILTKGEVQVSDKERHTQLEQMFRDIATIVADKCVNPETKRPYTVILIERAMKDIHYSVKPNKSTKQQALEVIKQLKEKMKIERAHMRLRFILPVNEGKKLKEKLKPLMKVVESEDYSQQLEIVCLIDPGCFREIDELIKKETKGRGSLEVLSLKDVEEGDEKFE

Approximately 30 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.