SCF 蛋白, Human (P.pastoris)

SCF Protein, Human (P.pastoris), the c-kit ligand, is a two disulfide bridge-containing cytokine in the regulation of the development and function of hematopoietic cell lineages and other cells such as mast cells, germ cells, and melanocytes.

重组人干细胞因子（rhSCF）蛋白特异性增强人髓系白血病细胞系TF-1的活力以及人肥大细胞系LAD2细胞的增殖和成熟。重组人 SCF (rhSCF) 可维持培养中的造血干细胞和肥大细胞，并在胚胎干细胞 (ESC)/诱导多能干细胞 (iPSC) 分化研究中得到广泛测试^[1]。

The ED₅₀ is <2 ng/mL as measured by human TF-1 cells, corresponding to a specific activity of 5 × 10⁵ units/mg.

Human

P. pastoris

Tag Free

P21583-1 (E26-A189)

4254  [NCBI]

EGICRNRVTNNVKDVTKLVANLPKDYMITLKYVPGMDVLPSHCWISEMVVQLSDSLTDLLDKFSNISEGLSNYSIIDKLVNIVDDLVECVKENSSKDLKKSFKSPEPRLFTPEEFFRIFNRSIDAFKDFVVASETSDCVVSSTLSPEKDSRVSVTKPFMLPPVA

Approximately 18.6 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against 10 mM acetic acid.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Akuta T, et al. Expression of bioactive soluble human stem cell factor (SCF) from recombinant Escherichia coli by coproduction of thioredoxin and efficient purification using arginine in affinity chromatography. Protein Expr Purif. 2015 Jan;105:1-7.  [Content Brief]