SCO1 蛋白, Human (GST)

The SCO1 protein is a copper metal chaperone that contributes to the maturation of cytochrome c oxidase subunit II. SCO1 Protein, Human (GST) is the recombinant human-derived SCO1 protein, expressed by E. coli , with N-GST labeled tag. The total length of SCO1 Protein, Human (GST) is 170 a.a., with molecular weight of ~20.40 kDa.

SCO1 蛋白作为铜金属伴侣，对于细胞色素 c 氧化酶亚基 II (MT-CO2/COX2) 的成熟至关重要。虽然SCO1不直接参与MT-CO2/COX2的合成，但在随后的成熟过程中稳定MT-CO2/COX2发挥着不可或缺的作用，促进铜转运至MT-CO2/COX2上的Cu(A)位点。除了在线粒体功能中的作用之外，SCO1 也是铜稳态的关键调节因子，影响铜转运蛋白 CTR1 的丰度和细胞膜定位。SCO1 作为同二聚体存在，与 COA6、COX20、COX18 和 SCO2 等各种伙伴相互作用，形成对于协调铜运输和利用至关重要的复合物。值得注意的是，SCO1 与 COX20、TMEM177 和 COX16 的相互作用受到复杂的调节，为了解参与线粒体铜稳态和细胞色素 C 氧化酶成熟的复杂蛋白质网络提供了见解。

Human

E. coli

N-GST

O75880 (G132-S301)

6341  [NCBI]

GKPLLGGPFSLTTHTGERKTDKDYLGQWLLIYFGFTHCPDVCPEELEKMIQVVDEIDSITTLPDLTPLFISIDPERDTKEAIANYVKEFSPKLVGLTGTREEVDQVARAYRVYYSPGPKDEDEDYIVDHTIIMYLIGPDGEFLDYFGQNKRKGEIAASIATHMRPYRKKS

Approximately 20.40 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 50 mM PB, 1 mM DTT, pH 7.2.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.