SDC4 蛋白, Mouse (HEK293, Fc)

SDC4 protein is a cell surface proteoglycan that cooperates with SDCBP and PDCD6IP to regulate exosome biogenesis.SDC4 exists as a homodimer and engages in important interactions with various intracellular partners.SDC4 Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived SDC4 protein, expressed by HEK293 , with C-hFc labeled tag.

Syndecan-4 (SDC4) 是一种细胞表面蛋白聚糖，与 SDCBP 和 PDCD6IP 合作，在调节外泌体生物发生中发挥着至关重要的作用。作为同二聚体，SDC4 参与各种蛋白质相互作用，包括与 CDCP1 和 SDCBP 结合，表明它参与多种细胞过程。通过其胞质结构域，SDC4 与 GIPC（特别是通过 PDZ 结构域）以及 NUDT16L1 形成相互作用。这些关联凸显了 SDC4 的多功能性质，表明它参与复杂的细胞信号传导途径和外泌体动力学的调节。

1.Measured by its binding ability in a functional ELISA.Immobilized human MDK at 10 μg/mL (100 μl/well) can bind mouse SDC4-Fc with a linear range of 0.16-1.25 μg/mL.
2.Measured by the ability of the immobilized protein to support the adhesion of A431 human epithelial carcinoma cells. When 5 x 10⁴ cells/well are added to mouse Syndecan-4 and human Fibronectin (0.5 μg/mL) coated plates, cell adhesion is enhanced in a dose dependent manner after 45 minutes at 37 °C. The ED₅₀ for this effect is 2.984 μg/mL, corresponding to a specific activity is 3.35×10² U/mg.

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  Measured by the ability of the immobilized protein to support the adhesion of A431 human epithelial carcinoma cells. When 5 x 10⁴ cells/well are added to mouse Syndecan-4 and human Fibronectin (0.5 μg/mL) coated plates, cell adhesion is enhanced in a dose dependent manner after 45 minutes at 37 °C. The ED₅₀ for this effect is 2.984 μg/mL, corresponding to a specific activity is 3.35×10² U/mg.

Mouse

HEK293

C-hFc

O35988 (E24-V146)

20971  [NCBI]

ESIRETEVIDPQDLLEGRYFSGALPDDEDAGGSDDFELSGSGDLDDTEEPRPFPEVIEPLVPLDNHIPENAQPGIRVPSEPKELEENEVIPKRAPSDVGDDMSNKVSMSSTAQGSNIFERTEV

Approximately 50-55 kDa due to the glycosylation.

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.