Semaphorin-4D/SEMA4D 蛋白, Rhesus Macaque (HEK293, His)

Semaphorin-4D/SEMA4D protein, a receptor for PLXNB1 and PLXNB2, regulates cell-cell signaling. It promotes inhibitory synapse development via PLXNB1, modulates neurite arborization through RHOA activation, and enhances viability of B-cells. SEMA4D induces endothelial cell migration via PTK2B/PYK2, SRC, and PI3K-AKT pathway. It forms homodimers and interacts with PLXNB2 and PLXNB1. Semaphorin-4D/SEMA4D Protein, Rhesus Macaque (HEK293, His) is the recombinant Rhesus Macaque-derived Semaphorin-4D/SEMA4D protein, expressed by HEK293 , with C-His labeled tag.

Semaphorin-4D/SEMA4D 蛋白是 PLXNB1 和 PLXNB2 的细胞表面受体，在细胞间信号传导中发挥着至关重要的作用。它参与调节 GABA 能突触发育，并以 PLXNB1 依赖性方式促进抑制性突触的发育。通过激活 PLXNB1 并与其相互作用，SEMA4D 调节海马神经元发育中神经突的复杂性和树枝化，介导 RHOA 的激活。此外，SEMA4D 还能促进小脑颗粒细胞的迁移并诱导 B 细胞聚集，从而增强其体外活力。在免疫系统中，SEMA4D 通过激活 PTK2B/PYK2、SRC 和磷脂酰肌醇 3-激酶-AKT 途径，在诱导内皮细胞迁移中发挥作用。它形成同二聚体并与 PLXNB2 相互作用，并与 PLXNB1 相互作用。

Rhesus Macaque

HEK293

C-His

EHH24221.1 (M22-R734)

/

Approximately 80.7 kDa.

Greater than 90% as determined by reducing SDS-PAGE

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.