Semaphorin-5A/SEMA5A 蛋白, Human (HEK293, His)

Semaphorin-5A/SEMA5A proteins act as bifunctional axon guidance signals, exerting attraction via heparan sulfate proteoglycans and inhibitory effects via chondroitin sulfate proteoglycans. As a ligand for the PLXNB3 receptor, SEMA5A disrupts glioma cell migration and invasion and induces cell collapse. Semaphorin-5A/SEMA5A Protein, Human (HEK293, His) is the recombinant human-derived Semaphorin-5A/SEMA5A protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Semaphorin-5A/SEMA5A Protein, Human (HEK293, His) is 743 a.a., with molecular weight of ~100.0 kDa.

Semaphorin-5A/SEMA5A 蛋白作为受硫酸化蛋白聚糖调节的双功能轴突导向信号发挥作用，通过与硫酸乙酰肝素蛋白聚糖 (HSPG) 的相互作用表现出有吸引力的效果，并通过与硫酸软骨素蛋白聚糖 (CSPG) 的相互作用表现出抑制作用。作为受体 PLXNB3 的配体，SEMA5A 在神经胶质瘤细胞中发挥着至关重要的作用，其对 PLXNB3 的刺激导致 F-肌动蛋白应力纤维解体、粘着斑破坏、细胞塌陷以及通过 AHGDIA 抑制细胞迁移和侵袭。- RAC1 介导的失活。此外，SEMA5A 可能通过增强内皮细胞增殖和迁移同时抑制细胞凋亡来促进血管生成。SEMA5A 与 PLXNB3 的结合强调了它参与多种细胞过程，为了解其在正常发育和病理条件下的潜在作用提供了宝贵的见解，特别是在轴突引导、细胞迁移和血管生成方面。

Human

HEK293

C-6*His

Q13591 (E23-T765)

9037  [NCBI]

EAQGTTQCQRTEHPVISYKEIGPWLREFRAKNAVDFSQLTFDPGQKELVVGARNYLFRLQLEDLSLIQAVEWECDEATKKACYSKGKSKEECQNYIRVLLVGGDRLFTCGTNAFTPVCTNRSLSNLTEIHDQISGMARCPYSPQHNSTALLTAGGELYAATAMDFPGRDPAIYRSLGILPPLRTAQYNSKWLNEPNFVSSYDIGNFTYFFFRENAVEHDCGKTVFSRAARVCKNDIGGRFLLEDTWTTFMKARLNCSRPGEVPFYYNELQSTFFLPELDLIYGIFTTNVNSIAASAVCVFNLSAIAQAFSGPFKYQENSRSAWLPYPNPNPHFQCGTVDQGLYVNLTERNLQDAQKFILMHEVVQPVTTVPSFMEDNSRFSHVAVDVVQGREALVHIIYLATDYGTIKKVRVPLNQTSSSCLLEEIELFPERRREPIRSLQILHSQSVLFVGLREHVVKIPLKRCQFYRTRSTCIGAQDPYCGWDVVMKKCTSLEESLSMTQWEQSISACPTRNLTVDGHFGVWSPWTPCTHTDGSAVGSCLCRTRSCDSPAPQCGGWQCEGPGMEIANCSRNGGWTPWTSWSPCSTTCGIGFQVRQRSCSNPTPRHGGRVCVGQNREERYCNEHLLCPPHMFWTGWGPWERCTAQCGGGIQARRRICENGPDCAGCNVEYQSCNTNPCPELKKTTPWTPWTPVNISDNGGHYEQRFRYTCKARLADPNLLEVGRQRIEMRYCSSDGTSGCST

Approximately 100.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.