Semaphorin-7A/SEMA7A 蛋白, Mouse (HEK293, C-6His)

Semaphorin-7A/SEMA7A proteins critically regulate integrin-mediated signaling, affecting cell migration and immune responses. It promotes focal adhesion complex formation, activates PTK2/FAK1, and leads to the phosphorylation of MAPK1 and MAPK3. Semaphorin-7A/SEMA7A Protein, Mouse (HEK293, C-6His) is the recombinant mouse-derived Semaphorin-7A/SEMA7A, expressed by HEK293 , with C-6*His labeled tag. ,

Semaphorin-7A/SEMA7A 蛋白在整合素介导的信号传导中发挥着至关重要的作用，有助于调节细胞迁移和免疫反应。它促进粘着斑复合物的形成并激活蛋白激酶PTK2/FAK1，导致随后MAPK1和MAPK3磷酸化。此外，它还能增强单核细胞和巨噬细胞促炎细胞因子的产生。这种蛋白质还在调节炎症和 T 细胞介导的免疫反应中发挥着重要作用。此外，它还能促进胚胎嗅球的轴突生长，并有助于黑素细胞的附着、扩散和树突生长。它与 PLXNC1 以及整合素 ITGA1 和 ITGB1 相互作用。

Measured by the ability of the immobilized protein to support the adhesion of THP‑1 human acute monocytic leukemia cells. When 5×10⁴ cells/well are added to coated plates, cell adhesion is enhanced in a dose dependent manner after 60 minutes at 37 °C. The ED₅₀ for this effect is 0.2644 μg/mL, corresponding to a specific activity is 3.782×10³ U/mg.

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  Measured by the ability of the immobilized protein to support the adhesion of THP‑1 human acute monocytic leukemia cells. When 5×10⁴ cells/well are added to coated plates, cell adhesion is enhanced in a dose dependent manner after 60 minutes at 37 °C. The ED₅₀ for this effect is 0.2644 μg/mL, corresponding to a specific activity is 3.782×10³ U/mg.

Mouse

HEK293

C-6*His

Q9QUR8 (Q45-A646)

20361

QGHSRSGPRISAVWKGQDHVDFSQPEPHTVLFHEPGSFSVWVGGRGKVYHFNFPEGKNASVRTVNIGSTKGSCQDKQDCGNYITLLERRGNGLLVCGTNARKPSCWNLVNDSVVMSLGEMKGYAPFSPDENSLVLFEGDEVYSTIRKQEYNGKIPRFRRIRGESELYTSDTVMQNPQFIKATIVHQDQAYDDKIYYFFREDNPDKNPEAPLNVSRVAQLCRGDQGGESSLSVSKWNTFLKAMLVCSDAATNRNFNRLQDVFLLPDPSGQWRDTRVYGVFSNPWNYSAVCVYSLGDIDRVFRTSSLKGYHMGLPNPRPGMCLPKKQPIPTETFQVADSHPEVAQRVEPMGPLKTPLFHSKYHYQKVVVHRMQASNGETFHVLYLTTDRGTIHKVVESGDQDHSFVFNIMEIQPFHRAAAIQAISLDADRRKLYVTSQWEVSQVPLDMCEVYSGGCHGCLMSRDPYCGWDQDRCVSIYSSQRSVLQSINPAEPHRECPNPKPDEAPLQKVSLARNSRYYLTCPMESRHATYLWRHEENVEQSCEPGHQSPSCILFIENLTARQYGHYRCEAQEGSYLREAQHWELLPEDRALAEQLMGHARALA

Approximately 75-90 kDa due to the glycosylation.

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.