Serpin A3C 蛋白, Mouse (HEK293, His)

Serpin A3C Protein, with its reactive center loop (RCL), guides interactions with target proteases.Binding leads to protease cleavage at the RCL, forming a stable serpin-protease complex, inactivating the protease.Variability in Serpin A3C's RCL sequences among Serpina3 paralogs may influence the specificity of target protease interactions, showcasing the protein's adaptability.Serpin A3C Protein, Mouse (HEK293, His) is the recombinant mouse-derived Serpin A3C protein, expressed by HEK293 , with C-His labeled tag.

Serpin A3C 蛋白具有从其主体突出的反应中心环 (RCL)，引导其与目标蛋白酶相互作用。结合后，蛋白酶在 RCL 内的反应位点裂解丝氨酸蛋白酶抑制剂，形成持久且共价的丝氨酸蛋白酶抑制剂-蛋白酶复合物。该复合物以蛋白酶失活为标志，表现出显着的稳定性。Serpina3 旁系同源物的 RCL 内的不同序列导致变异性，可能影响靶蛋白酶相互作用的特异性。

Measured by its binding ability in a functional ELISA. When Recombinant Mouse SerpinA3C is present at 10 μg/mL, can bind Anti-SerpinA3c Antibody. The ED₅₀ for this effect is 5.374 ng/mL.

Mouse

HEK293

C-His

P29621 (D23-A417)

16625  [NCBI]

DGTLERDTLFHKDKENGTQLDSLTLASINTDFAFSLYKKLALKNPDTNIVFSPLSISAALAIVSLGAKGNTLEEILEGLNFNLTETPEADIHQGFGHLLQRLSHPGEQVQISTGSALFVEKHLQILAEFQEKARALYQAEAFTADFQQPLEATKLINDYVSNQTQRKIKGLISDLDTDTLMVLVNYIYFKGKWKMPFNPRDTFESEFYLDVKRSVKVPMMKIKTLTTPYFRDEELSCTVVELKYKGNASALFILPDQGRMQQVEASLQPETLRKWKNSLRPRKMGELYLPKFSISTDYSLKNILPELGIKEIFSKQADLSGITGTKDLIVSQMVHKAVLDVAETGTEGVAATGVNFRILSRRTSLWFNRTFLMVISHTDVQTTLFIAKITHPKRA

Approximately 55-70 & 115 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.