Serpin F1 蛋白, Human (HEK293, N-His)

Serpin F1 protein, a neurotrophic factor, crucially promotes extensive neuronal differentiation in retinoblastoma cells and serves as a potent inhibitor of angiogenesis. Unlike active serpins, Serpin F1 does not undergo the typical S (stressed) to R (relaxed) conformational transition, lacking serine protease inhibitory activity. It also interacts with PNPLA2, enhancing the phospholipase A2 activity of PNPLA2. Serpin F1 Protein, Human (HEK293, N-His) is the recombinant human-derived Serpin F1 protein, expressed by HEK293 , with N-6*His labeled tag. The total length of Serpin F1 Protein, Human (HEK293, N-His) is 399 a.a., with molecular weight of ~49.37 kDa.

神经营养蛋白 Serpin F1 在促进视网膜母细胞瘤细胞广泛神经元分化中发挥关键作用。此外，它还可以作为血管生成的有效抑制剂。值得注意的是，丝氨酸蛋白酶抑制剂 F1 不会经历活性丝氨酸蛋白酶抑制剂典型的 S（应激）到 R（松弛）构象转变，导致缺乏丝氨酸蛋白酶抑制活性。此外，它与 PNPLA2 相互作用，促进 PNPLA2 的磷脂酶 A2 活性。

Measured by its ability to enhance the adhesion of Saos-2 human osteosarcoma cells to bovine Collagen I coated plate. The ED₅₀ this effect is 0.2506 μg/mL, corresponding to a specific activity is 3.99×10³ units/mg.

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  Measured by its ability to enhance the adhesion of Saos-2 human osteosarcoma cells to bovine Collagen I coated plate. The ED₅₀ for this effect is 0.2506 μg/ml, corresponding to a specific activity is 3.99×10³ units/mg.

Human

HEK293

N-6*His

P36955 (Q20-P418)

5176  [NCBI]

QNPASPPEEGSPDPDSTGALVEEEDPFFKVPVNKLAAAVSNFGYDLYRVRSSTSPTTNVLLSPLSVATALSALSLGAEQRTESIIHRALYYDLISSPDIHGTYKELLDTVTAPQKNLKSASRIVFEKKLRIKSSFVAPLEKSYGTRPRVLTGNPRLDLQEINNWVQAQMKGKLARSTKEIPDEISILLLGVAHFKGQWVTKFDSRKTSLEDFYLDEERTVRVPMMSDPKAVLRYGLDSDLSCKIAQLPLTGSMSIIFFLPLKVTQNLTLIEESLTSEFIHDIDRELKTVQAVLTVPKLKLSYEGEVTKSLQEMKLQSLFDSPDFSKITGKPIKLTQVEHRAGFEWNEDGAGTTPSPGLQPAHLTFPLDYHLNQPFIFVLRDTDTGALLFIGKILDPRGP

Approximately 49.37 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB,150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.