sFRP-3 蛋白, Mouse (HEK293, His)

The sFRP-3 protein is a soluble Frizzled-related protein that modulates Wnt signaling by directly interacting with Wnt, thereby regulating cell growth and differentiation. Known as FRZB, it acts as a Wnt8 signaling antagonist with crucial effects on limb skeletogenesis. sFRP-3 Protein, Mouse (HEK293, His) is the recombinant mouse-derived sFRP-3 protein, expressed by HEK293 , with C-6*His labeled tag.

sFRP-3 是一种可溶性卷曲相关蛋白，通过与 Wnt 的直接相互作用作为 Wnt 信号传导的调节剂，有助于调节特定细胞类型的细胞生长和分化。值得注意的是，SFRP-3（也称为 FRZB）在肢体骨骼发生中发挥关键作用，作为 Wnt8 信号传导的拮抗剂。其调节影响延伸至软骨细胞成熟和长骨发育。除了参与 Wnt 信号传导调节之外，SFRP-3 还与 MYOC 相互作用，展示了其参与与细胞过程相关的分子相互作用。这些不同的功能强调了 sFRP-3 在 Wnt 介导的途径和骨骼发生中的多方面调节作用。

Measured by its ability to inhibit proliferation in MC3T3-E1 mouse preosteoblast cells. The ED50 for this effect is 5.797 µg/mL.

Mouse

HEK293

C-6*His

P97401 (A33-S323)

20378

AACEPVRIPLCKSLPWNMTKMPNHLHHSTQANAILAMEQFEGLLGTHCSPDLLFFLCAMYAPICTIDFQHEPIKPCKSVCERARQGCEPILIKYRHSWPESLACDELPVYDRGVCISPEAIVTADGADFPMDSSTGHCRGASSERCKCKPVRATQKTYFRNNYNYVIRAKVKEVKMKCHDVTAVVEVKEILKASLVNIPRDTVNLYTTSGCLCPPLTVNEEYVIMGYEDEERSRLLLVEGSIAEKWKDRLGKKVKRWDMKLRHLGLGKTDASDSTQNQKSGRNSNPRPARS

Approximately 35-37 kDa due to the glycosylation.

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in PBS. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.