SFRP2 蛋白, Human (HEK293, His)

SFRP2 Protein is a soluble crimson-associated protein that promotes tumor development by activating the Wnt/β-catenin signaling pathway. The methylation of SFRP2 Protein DNA is associated with tumor development. SFRP2 Protein, Human (HEK293, His) is the recombinant human-derived SFRP2 protein, expressed by HEK293 , with C-His labeled tag. The total length of SFRP2 Protein, Human (HEK293, His) is 271 a.a., with molecular weight of 33 kDa.

Secreted frizzled-related protein 2 (SFRP2) 是一种可溶性卷曲相关蛋白，通过 DNA 去甲基化调控激活 Wnt/β-catenin 信号通路。SFRP2 含有一个富含半胱氨酸的结构域，是 Wnt 信号的可溶性调节剂。SFRP2 可将人诱导多能干细胞分化为成熟心肌细胞。SFRP2 过表达可诱导前列腺癌细胞成骨细胞样表型。SFRP2 通过激活 Wnt/β-catenin 信号通路促进胶质瘤细胞的癌变和放射耐药。SFRP2 启动子在多种恶性肿瘤中被发现高甲基化 在多种肿瘤细胞中过表达，可能与肿瘤发生相关。SFRP2 可作为 HBV 相关肝细胞癌的非侵入性生物标志物。

Measured by its ability to synergize with Wnt-3a to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED₅₀ for this effect is 28.27 ng/mL in the presence of 20 ng/mL of Recombinant Mouse Wnt-3a, corresponding to a specific activity is 3.54×10⁴ units/mg.

Human

HEK293

C-6*His

Q96HF1 (L25-C295)

6423

LFLFGQPDFSYKRSNCKPIPANLQLCHGIEYQNMRLPNLLGHETMKEVLEQAGAWIPLVMKQCHPDTKKFLCSLFAPVCLDDLDETIQPCHSLCVQVKDRCAPVMSAFGFPWPDMLECDRFPQDNDLCIPLASSDHLLPATEEAPKVCEACKNKNDDDNDIMETLCKNDFALKIKVKEITYINRDTKIILETKSKTIYKLNGVSERDLKKSVLWLKDSLQCTCEEMNDINAPYLVMGQKQGGELVITSVKRWQKGQREFKRISRSIRKLQC

Approximately 33 kDa

Greater than 95% as determined by Tris-Bis PAGE.

无菌粉末

Lyophilized from a 0.22 μm filtered solution of PBS, pH 6.2, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.