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  3. SH3PXD2A 蛋白, Human (Myc, His)

SH3PXD2A 蛋白, Human (Myc, His)

目录号: HY-P71572
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SH3PXD2A是一种衔接蛋白,在侵袭伪足和足体的形成中起着至关重要的作用,从而增强癌细胞的侵袭力。它与 ADAM、NOX 和磷酸肌醇相互作用,并参与多种细胞过程。SH3PXD2A 蛋白, Human (Myc, His) 是重组的 SH3PXD2A 蛋白,由 E. coli 表达,带有 N-His, C-Myc 标签。SH3PXD2A 蛋白, Human (Myc, His) 全长 85 个氨基酸,分子量约为 ~14.0 kDa。

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描述

SH3PXD2A is an adapter protein that plays a crucial role in the formation of invadopodia and podosomes, thereby enhancing the invasiveness of cancer cells. It interacts with ADAM, NOX and phosphoinositides and contributes to a variety of cellular processes. SH3PXD2A Protein, Human (Myc, His) is the recombinant human-derived SH3PXD2A protein, expressed by E. coli , with N-His, C-Myc labeled tag. The total length of SH3PXD2A Protein, Human (Myc, His) is 85 a.a., with molecular weight of ~14.0 kDa.

研究背景

SH3PXD2A 作为衔接蛋白,在侵袭足和足体的形成中发挥关键作用,有助于细胞外基质降解并增强某些癌细胞的侵袭性。这种多面蛋白质与基质金属蛋白酶 (ADAM)、NADPH 氧化酶 (NOX) 和磷酸肌醇建立联系,从而参与多种细胞过程。作为组织者蛋白,SH3PXD2A 促进 NOX1 或 NOX3 依赖性活性氧 (ROS) 的生成,并确保精确的 ROS 定位。它与 ADAM12 协同作用,介导 β 淀粉样肽诱导的神经毒性作用。该蛋白进一步与 CYBA、ADAM15、ADAM19、NOXO1 和 NOXA1 相互作用,形成复杂的分子关联网络。值得注意的是,它与 FASLG 的相互作用强调了它参与复杂的细胞信号传导途径。

种属

Human

表达系统

E. coli

标签

N-His;C-Myc

蛋白编号

Q5TCZ1 (902P-986P)

基因 ID
蛋白结构
N-term
10*His
SH3PXD2A (902P-986P)
Accession # Q5TCZ1
Myc
C-term
中文名
SH3PXD2A 蛋白
同用名
Adapter protein TKS5; Five SH3 domain-containing protein; SH3 and PX domain-containing protein 2A; SH3 multiple domains protein 1; Sh3md1; Sh3pxd2a; SPD2A_HUMAN; TKs5; Tyrosine kinase substrate with five SH3 domains
氨基酸序列

PDPSGKELDTVPAKGRQNEGKSDSLEKIERRVQALNTVNQSKKATPPIPSKPPGGFGKTSGTPAVKMRNGVRQVAVRPQSVFVSP

分子量

Approximately 14.0 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

SH3PXD2A Protein, Human (Myc, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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SH3PXD2A Protein, Human (Myc, His)
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