2. 重组蛋白
  3. Enzymes & Regulators Ubiquitin Related Proteins
  4. Transferases (EC 2) Ubiquitin Enzymes
  5. E3 Ligases
  6. SH3RF3 蛋白, Human (His)

SH3RF3 蛋白, Human (His)

目录号: HY-P701569
产品使用指南

SH3RF3蛋白是一种E3泛素蛋白连接酶，在介导泛素向靶蛋白转移、影响蛋白降解、细胞信号传导和各种生物过程中发挥关键作用。作为一种 E3 连接酶，SH3RF3 可能促进泛素介导的特定底物蛋白的蛋白酶体降解，从而影响其细胞水平和活性。SH3RF3 蛋白, Human (His) 是重组的 SH3RF3 蛋白，由 E. coli 表达，带有 N-6*His 标签。SH3RF3 蛋白, Human (His) 全长 881 个氨基酸。

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100 μg   询价  

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描述

SH3RF3 protein is an E3 ubiquitin protein ligase that plays a key role in mediating the transfer of ubiquitin to target proteins, affecting protein degradation, cell signaling, and various biological processes. As an E3 ligase, SH3RF3 may promote ubiquitin-mediated proteasomal degradation of specific substrate proteins, thereby affecting their cellular levels and activity. SH3RF3 Protein, Human (His) is the recombinant human-derived SH3RF3 protein, expressed by E. coli , with N-6*His labeled tag. The total length of SH3RF3 Protein, Human (His) is 881 a.a., .
研究背景

SH3RF3 蛋白作为 E3 泛素蛋白连接酶发挥作用，在介导泛素分子向靶蛋白的转移中发挥关键作用。这种酶活性有助于调节蛋白质降解、细胞信号传导和各种生物过程。作为一种 E3 连接酶，SH3RF3 可能促进泛素介导的特定底物蛋白的蛋白酶体降解，从而影响其细胞水平和活性。SH3RF3 介导的泛素化的精确靶标和下游效应是了解该蛋白在细胞途径和分子机制中的功能意义的重要领域。
种属

Human
表达系统

E. coli
标签

N-6*His
蛋白编号

Q8TEJ3 (L2-F882)
基因 ID

344558
蛋白结构
N-term
6*His
SH3RF3 (L2-F882)
Accession # Q8TEJ3
C-term
同用名
SH3RF3; E3 ubiquitin-protein ligase SH3RF3; Plenty of SH3s 2; SH3 domain-containing RING finger protein 3; SH3 multiple domains protein 4
纯度

Greater than 90% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

SH3RF3 Protein, Human (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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