Siglec-15 蛋白, Mouse (HEK293, His)

Siglec-15 Protein, a Siglec family member and type-1 transmembrane protein, is constitutively expressed in osteoclasts, macrophages and dendritic cells. Siglec-15 plays a pivotal role in the development and differentiation of osteoclastogenesis, inhibiting antigen-specific T cell responses in vitro and in vivo. Siglec-15 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Siglec-15 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Siglec-15 Protein, Mouse (HEK293, His) is 239 a.a., with molecular weight of 30-40 kDa.

Siglec-15 是 Siglec 家族的成员和 1 型跨膜蛋白，在破骨细胞、巨噬细胞和树突状细胞中组成型表达。Siglec-15 在肌动蛋白细胞骨架组织的上游或调控范围内发挥作用。Siglec-15缺失可以促进骨形成并减少骨吸收，表明Siglec-15在破骨细胞生成的发育和分化中起着关键作用，可能作为抑制骨吸收和促进骨量增加的骨重塑的靶点。Siglec-15 是一种主要由巨噬细胞介导的 T 细胞反应抑制剂。在肿瘤中，Siglec-15 受到 IFN-γ 的负向调节，从而影响效应 T 细胞介导的抗肿瘤免疫。Siglec-15 的基因消融或抗体阻断可增强 TME 中的抗肿瘤免疫力，并抑制某些小鼠模型中的肿瘤生长。Siglec-15 可作为正常化癌症免疫治疗的潜在靶点^[1][2][3][4]。

Immobilized Mouse Siglec-15-His at 2μg/ml (100 μl/well) can bind Anti-Human Siglec15 mAb, with special bioactivity: 69.39ng/mL.

Mouse

HEK293

C-6*His

A7E1W8 (R24-T262)

620235  [NCBI]

RRDASGDLLNTEAHSAPAQRWSMQVPAEVNAEAGDAAVLPCTFTHPHRHYDGPLTAIWRSGEPYAGPQVFRCTAAPGSELCQTALSLHGRFRLLGNPRRNDLSLRVERLALADSGRYFCRVEFTGDAHDRYESRHGVRLRVTAAAPRIVNISVLPGPAHAFRALCTAEGEPPPALAWSGPAPGNSSAALQGQGHGYQVTAELPALTRDGRYTCTAANSLGRAEASVYLFRFHGAPGTST

30-40 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, 150 mM NaCl, 5% Thehalose, 0.3% Chaps, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.