Siglec-3/CD33 蛋白, Cynomolgus/Rhesus Macaque (HEK293, His)

Siglec-3/CD33, a sialic-acid-binding lectin, crucially mediates cell-cell interactions and immune cell quiescence. Preferring sialic acid on specific mucins, it forms homodimers, interacting with PTPN6/SHP-1 and PTPN11/SHP-2 upon phosphorylation. CD33 also engages C1QA through its C-terminus, activating CD33 inhibitory motifs. Siglec-3/CD33 Protein, Cynomolgus/Rhesus Macaque (HEK293, His) is the recombinant Rhesus Macaque-derived Siglec-3/CD33 protein, expressed by HEK293 , with C-His labeled tag.

Siglec-3/CD33 是一种唾液酸结合免疫球蛋白样凝集素，在介导细胞间相互作用和维持免疫细胞处于静息状态方面发挥着至关重要的作用。它表现出优先将唾液酸结合到特定粘蛋白的短 O 连接聚糖上。该蛋白通过二硫键形成同二聚体，并在磷酸化后与信号分子（如 PTPN6/SHP-1 和 PTPN11/SHP-2）相互作用。此外，CD33 通过其 C 末端与 C1QA 结合，导致 CD33 抑制基序的激活。

Immobilized Human CD33 at 2 μg/mL (100 μL/well) can bind Anti-CD33 Antibody. The ED₅₀ for this effect is 22.60 ng/mL.

Rhesus Macaque

HEK293

C-His

A0A2K5W2R5 (M16-G248)

102117580  [NCBI]

MDPRVRLEVQESVTVQEGLCVLVPCTFFHPVPYHTRNSPVHGYWFREGAIVSLDSPVATNKLDQEVREETQGRFRLLGDPSRNNCSLSIVDARRRDNGSYFFRMEKGSTKYSYKSTQLSVHVTDLTHRPQILIPGALDPDHSKNLTCSVPWACEQGTPPIFSWMSAAPTSLGLRTTHSSVLIITPRPQDHGTNLTCQVKFPGAGVTTERTIQLNVSYASQNPRTDIFLGDGSG

Approximately 45.2 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.