Siglec-5 蛋白, Human (HEK293, Fc)

Siglec-5 Protein, a putative adhesion molecule, participates in sialic-acid dependent cellular binding, showing equal affinity for alpha-2,3-linked and alpha-2,6-linked sialic acid. Its sialic acid recognition site may be masked through cis interactions with sialic acids on the same cell surface. Siglec-5 Protein, Human (HEK293, Fc) is the recombinant human-derived Siglec-5 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of Siglec-5 Protein, Human (HEK293, Fc) is 418 a.a..

Siglec-5 蛋白是一种假定的粘附分子，与唾液酸依赖性细胞结合有关，对 α-2,3-连接和 α-2,6-连接唾液酸显示出相同的亲和力。值得注意的是，Siglec-5 的唾液酸识别位点可能通过与同一细胞表面上的唾液酸的顺式相互作用而受到掩蔽。

Measured by its binding ability in a functional ELISA. Immobilized Siglec-5 at 1 μg/mL (100 μL/well) can bind Anti Siglec-5 antibody. The ED₅₀ for this effect is 0.7241 μg/mL.

Human

HEK293

C-hFc

O15389 (E17-T434)

8778  [NCBI]

EKPVYELQVQKSVTVQEGLCVLVPCSFSYPWRSWYSSPPLYVYWFRDGEIPYYAEVVATNNPDRRVKPETQGRFRLLGDVQKKNCSLSIGDARMEDTGSYFFRVERGRDVKYSYQQNKLNLEVTALIEKPDIHFLEPLESGRPTRLSCSLPGSCEAGPPLTFSWTGNALSPLDPETTRSSELTLTPRPEDHGTNLTCQMKRQGAQVTTERTVQLNVSYAPQTITIFRNGIALEILQNTSYLPVLEGQALRLLCDAPSNPPAHLSWFQGSPALNATPISNTGILELRRVRSAEEGGFTCRAQHPLGFLQIFLNLSVYSLPQLLGPSCSWEAEGLHCRCSFRARPAPSLCWRLEEKPLEGNSSQGSFKVNSSSAGPWANSSLILHGGLSSDLKVSCKAWNIYGSQSGSVLLLQGRSNLGT

Approximately 90-120 kDa due to the glycosylation.

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 8.0 or PBS, pH 6.5, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.