1. 重组蛋白
  2. CD Antigens Receptor Proteins
  3. Dendritic Cell CD Proteins Siglec
  4. Siglec-5/CD170 Siglec-5/CD170
  5. Siglec-5 蛋白, Mouse (HEK293, His)

Siglec-5 蛋白, Mouse (HEK293, His)

目录号: HY-P78354
COA 产品使用指南

Siglec-F 蛋白是一种假定的粘附分子,关键介导唾液酸依赖性与细胞的结合,对 α-2,3 连接的唾液酸具有优先亲和力。值得注意的是,其唾液酸识别位点可能通过与同一细胞表面上的唾液酸的顺式相互作用而被隐藏。Siglec-5 蛋白, Mouse (HEK293, His) 是重组的 Siglec-5 蛋白,由 HEK293 表达,带有 C-His 标签。Siglec-5 蛋白, Mouse (HEK293, His) 全长 423 个氨基酸,分子量为 65-68 kDa。

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规格 价格 是否有货 数量
20 μg ¥1700 In-stock
50 μg ¥3100 In-stock
100 μg ¥4950 In-stock
> 100 μg   询价  

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描述

Siglec-F Protein, a putative adhesion molecule, crucially mediates sialic-acid dependent binding to cells with a preferential affinity for alpha-2,3-linked sialic acid. Notably, its sialic acid recognition site may be concealed through cis interactions with sialic acids on the same cell surface. Siglec-5 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Siglec-5 protein, expressed by HEK293 , with C-His labeled tag. The total length of Siglec-5 Protein, Mouse (HEK293, His) is 423 a.a., with molecular weight of 65-68 kDa.

研究背景

Siglec-F 蛋白被认为是一种假定的粘附分子,在介导唾液酸依赖性细胞结合中发挥着至关重要的作用。它对 α-2,3-连接的唾液酸表现出优先亲和力。值得注意的是,Siglec-F 的唾液酸识别位点可能由于与同一细胞表面上存在的唾液酸的顺式相互作用而被隐藏。

种属

Mouse

表达系统

HEK293

标签

C-His

蛋白编号

Q920G3 (T17-L439)

基因 ID
蛋白结构
N-term
Siglec-5 (T17-L439)
Accession # Q920G3
His
C-term
同用名
CD170; CD33 antigen-like 2; CD33L2; OBBP2; Siglec5; SIGLEC5; OB-BP2
分子量

65-68 kDa

纯度

Greater than 95% as determined by Tris-Bis PAGE.

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Siglec-5 Protein, Mouse (HEK293, His)
目录号:
HY-P78354
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