Siglec-7 蛋白, Human (HEK293, His)

Siglec-7 protein is a putative adhesion molecule that mediates sialic acid-dependent cell binding with preference for α-2,3- and α-2,6-linked sialic acids.It interacts with disialoganglioside and may be masked by cis interactions.Siglec-7 Protein, Human (HEK293, His) is the recombinant human-derived Siglec-7 protein, expressed by HEK293 , with C-6*His labeled tag.

Siglec-7 蛋白被鉴定为假定的粘附分子，充当唾液酸依赖性细胞结合的介质。它优先结合 α-2,3- 和 α-2,6- 连接的唾液酸，并与二唾液酸神经节苷脂相互作用，例如二唾液酸半乳糖基球苷、二唾液酸乳四糖神经酰胺和二唾液酸 GalNAc 乳四糖神经酰胺。值得注意的是，Siglec-7 的唾液酸识别位点可能由于与同一细胞表面上的唾液酸的顺式相互作用而被掩蔽。在免疫反应中，它充当抑制性受体，在配体结合时诱导酪氨酸磷酸化，并通过其 SH2 结构域招募细胞质磷酸酶，从而阻断信号转导并抑制自然杀伤细胞的细胞毒性。Siglec-7 还可能在造血作用中发挥作用，抑制 CD34+ 细胞前体向骨髓单核细胞谱系的分化，并在体外阻碍白血病骨髓细胞的增殖。此外，它在磷酸化后与 PTPN6/SHP-1 相互作用。

Human

HEK293

C-6*His

Q9Y286 (Q19-L353)

27036  [NCBI]

QKSNRKDYSLTMQSSVTVQEGMCVHVRCSFSYPVDSQTDSDPVHGYWFRAGNDISWKAPVATNNPAWAVQEETRDRFHLLGDPQTKNCTLSIRDARMSDAGRYFFRMEKGNIKWNYKYDQLSVNVTALTHRPNILIPGTLESGCFQNLTCSVPWACEQGTPPMISWMGTSVSPLHPSTTRSSVLTLIPQPQHHGTSLTCQVTLPGAGVTTNRTIQLNVSYPPQNLTVTVFQGEGTASTALGNSSSLSVLEGQSLRLVCAVDSNPPARLSWTWRSLTLYPSQPSNPLVLELQVHLGDEGEFTCRAQNSLGSQHVSLNLSLQQEYTGKMRPVSGVLL

50-70 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.