SIRP beta 蛋白, Mouse (HEK293, C-His)

SIRP-beta (Sirpb, CD172b) is a member of the signal-regulatory-protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are receptor-type transmembrane glycoproteins, Sirpb was found to interact with TYROBP/DAP12, a transmembrane adaptor protein bearing immunoreceptor tyrosine-based activation motifs, resulting in the tyrosine phosphorylation of DAP12 and the subsequent recruitment of Syk to the Sirpb-DAP12 complex. Sirpb is also implicated as a positive regulator of hematopoietic cells. SIRP beta Protein, Mouse (HEK293, His) is the recombinant mouse-derived SIRP beta protein, expressed by HEK293 , with C-His labeled tag.

SIRP- β (Sirpb, CD172b) 是信号调节蛋白 (SIRP) 家族的成员，也属于免疫球蛋白超家族 (IgSF)，其胞外结构域有三个 IgSF 结构域。SIRP 家族成员是受体型跨膜糖蛋白，已知参与受体酪氨酸激酶偶联信号传导过程的负调控。Sirpb 被发现与 TYROBP/DAP12 相互作用，TYROBP/DAP12 是一种携带免疫受体酪氨酸激活基序的跨膜衔接蛋白。在 RBL-2H3 细胞转染物中，Sirpb 的连接导致 DAP12 的酪氨酸磷酸化，随后 Syk 被募集到 Sirpb-DAP12 复合体上。它还刺激了这些细胞的血清素释放。因此，Sirpb 被认为是造血细胞的积极调节因子^[1][2]。

Measured by its binding ability in a functional ELISA. Immobilized Mouse SIRP beta at 1μg/mL (100 μL/well) can bind Human SP-D (HY-P70986). The ED₅₀ for this effect is 722.6 ng/mL.

Mouse

HEK293

C-10*His

BAD26610 (A27-K363)

/

AMRELKVIQPVKSFFVGAGGSATLNCTVTSLLPVGPMRWYRGIGQSRLLIYSFTGEGFPRITNTSDTTKRNNMDFSIRISNVTPADSGTYYCVKFQRGPSDFYTEIQSGGGTELSVLAKPSSPMVSGPAARAVPQQTVTFTCRSHGFFPRNLTLKWFKNGDEISHLETSVEPEETSVSYRVSSTVQVVLEPRDVRSQIICEVDHVTLDRAPLRGIAHISEFIQVPPTLEIRQQPTMVWNVINVTCQIQKFYPPSFQLTWLENGNISRREVPFTLTVNKDGTYNWISCLLVNISALEENMVVTCQVEHDGQAEVIETHTVVVTEHQRVKELKTAGIAK

Approximately 54-77 kDa due to the glycosylation.

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 5% trehalose, 5% mannitol and 0.01% Tween80.

<0.1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.