SLAMF2/CD48 蛋白, Rat (HEK293, His)

SLAMF2/CD48 is a GPI-anchored glycoprotein that regulates immune cells. It interacts with receptors like CD2 and CD244/2B4, activating LCK and LAT in T-cell signaling. SLAMF2 also phosphorylates CD244, leading to immunological synapse formation and targeted release of cytolytic granules by T-lymphocytes and NK-cells. Its direct interactions with CD2, CD244, and LCK contribute to immune response regulation. SLAMF2/CD48 Protein, Rat (HEK293, His) is the recombinant rat-derived SLAMF2/CD48 protein, expressed by HEK293 , with C-His labeled tag. The total length of SLAMF2/CD48 Protein, Rat (HEK293, His) is 194 a.a., with molecular weight of 32-50 kDa.

SLAMF2，也称为 CD48，是一种糖基磷脂酰肌醇 (GPI) 锚定的细胞表面糖蛋白，在免疫细胞调节中发挥着至关重要的作用。通过其 N 端免疫球蛋白结构域，SLAMF2 与细胞表面受体（例如 2B4/CD244 或 CD2）相互作用，以调节免疫细胞功能和激活。在 T 细胞信号转导中，SLAMF2 与 CD2 形成复合物，促进 Src 家族蛋白激酶 LCK 和 LAT 募集至 TCR/CD3 复合物。这种相互作用导致 LCK 磷酸化和随后的激活。此外，SLAMF2 诱导 CD244 的细胞质免疫受体酪氨酸开关基序 (ITSM) 磷酸化，启动信号传导事件，最终导致免疫突触的形成以及 T 淋巴细胞和 NK 细胞靶向释放含有穿孔素和颗粒酶的溶细胞颗粒。SLAMF2 与 CD2、CD244 和 LCK 建立直接相互作用，有助于其在免疫反应中的调节作用。

Measured by its binding ability in a functional ELISA. Immobilized 2B4/CD244 at 5μg/mL (100μL/well) can bind Rat SLAMF2. The ED₅₀ for this effect is 2.78 μg/mL.

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  Measured by its binding ability in a functional ELISA. Immobilized 2B4/CD244 at 5μg/mL (100μL/well) can bind Rat SLAMF2. The ED₅₀ for this effect is 2.78 μg/mL.

Rat

HEK293

C-6*His

P10252 (F23-R216)

245962  [NCBI]

FQDQSVPNVNAITGSNVTLTILKHPLASYQRLTWLHTTNQKILEYFPNGKKTVFESVFKDRVDLDKTNGALRIYNVSKEDRGDYYMRMLHETEDQWKITMEVYDLVSKPAIKIEKTKNLTDSCHLRLSCKVEDQGVDYTWYEDSGPFPQRNPGYVLEITITPHNKSTFYTCQVSNPVSSENDTLYFIPPCTLAR

Approximately 32-50 kDa 32-50 kDa in SDS-PAGE under reducing conditions due to the glycosylation.

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.