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  3. SLC11A1 蛋白, Human (Sf9, His, MBP, FLAG)

SLC11A1 蛋白, Human (Sf9, His, MBP, FLAG)

目录号: HY-P702016
产品使用指南

SLC11A1 蛋白是一种巨噬细胞特异性反向转运蛋白,可通过质子梯度可逆转运金属离子。它定位于晚期内体溶酶体膜并将二价阳离子转运到酸性隔室中,影响抗菌活性。SLC11A1 蛋白, Human (Sf9, His, MBP, FLAG) 是重组的 SLC11A1 蛋白,由 Sf9 insect cells 表达,带有 N-MBP, C-Flag, N-8*His 标签。SLC11A1 蛋白, Human (Sf9, His, MBP, FLAG) 全长 549 个氨基酸。

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描述

The SLC11A1 protein is a macrophage-specific antiporter that transports metal ions reversibly through proton gradients. It localizes to late endosomal lysosomal membranes and transports divalent cations into acidic compartments, affecting antibacterial activity. SLC11A1 Protein, Human (Sf9, His, MBP, FLAG) is the recombinant human-derived SLC11A1 protein, expressed by Sf9 insect cells , with N-MBP, C-Flag, N-8*His labeled tag. The total length of SLC11A1 Protein, Human (Sf9, His, MBP, FLAG) is 549 a.a., .

研究背景

SLC11A1 蛋白是一种巨噬细胞特异性反向转运蛋白,可促进逆质子梯度的双向金属离子转运。位于晚期内体溶酶体膜上,它将二价阳离子从胞质溶胶转移到这些酸性区室中,直接影响抗菌活性。它特别参与铁代谢,在宿主对细胞内寄生虫的先天抵抗力中发挥着至关重要的作用。这种抗性机制涉及隔离 Fe(2+) 和 Mn(2+),它们是原核和真核过氧化氢酶和超氧化物歧化酶的必需辅助因子。隔离有双重目的,保护巨噬细胞免受活性氧的影响,并阻止病原体合成其保护性酶所需的阳离子(可能)。

种属

Human

表达系统

Sf9 insect cells

标签

N-MBP;C-Flag;N-8*His

蛋白编号

P49279 (T2-G550)

基因 ID

6556

蛋白结构
N-term
8*His-MBP
SLC11A1 (T2-G550)
Accession # P49279
Flag
C-term
同用名
SLC11A1; Natural resistance-associated macrophage protein 1; NRAMP 1; Solute carrier family 11 member 1
纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

SLC11A1 Protein, Human (Sf9, His, MBP, FLAG) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
SLC11A1 Protein, Human (Sf9, His, MBP, FLAG)
目录号:
HY-P702016
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