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  3. SLC19A1 蛋白, Human (Sf9, His, Strep, FLAG)

SLC19A1 蛋白, Human (Sf9, His, Strep, FLAG)

目录号: HY-P702007
产品使用指南

SLC19A1 蛋白充当反向转运蛋白,通过输出有机阴离子来输入还原的叶酸和环状二核苷酸。它充当次级主动转运蛋白,输出细胞内有机阴离子以促进底物吸收。SLC19A1 蛋白, Human (Sf9, His, Strep, FLAG) 是重组的 SLC19A1 蛋白,由 Sf9 insect cells 表达,带有 N-Strep, C-Flag, N-8*His 标签。SLC19A1 蛋白, Human (Sf9, His, Strep, FLAG) 全长 590 个氨基酸。

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描述

The SLC19A1 protein acts as an antiporter, importing reduced folate and cyclic dinucleotides by exporting organic anions. It acts as a secondary active transporter, exporting intracellular organic anions to facilitate substrate uptake. SLC19A1 Protein, Human (Sf9, His, Strep, FLAG) is the recombinant human-derived SLC19A1 protein, expressed by Sf9 insect cells , with N-Strep, C-Flag, N-8*His labeled tag. The total length of SLC19A1 Protein, Human (Sf9, His, Strep, FLAG) is 590 a.a., .

研究背景

SLC19A1 蛋白作为反向转运蛋白,通过有机阴离子输出驱动的机制促进还原叶酸或环状二核苷酸子集的输入。作为次级主动转运蛋白,它沿着浓度梯度输出细胞内有机阴离子,以促进其底物的吸收。该蛋白对 N5-甲基四氢叶酸(叶酸的主要循环形式)具有高亲和力,并且可以介导抗叶酸药物甲氨蝶呤的输入。此外,它还是免疫反应性环状二核苷酸的输入者,包括环状 GMP-AMP (2'-3'-cGAMP),一种响应细胞质中 DNA 病毒而产生的免疫信使,及其连接异构体 3'-3'- cGAMP,在触发更大的免疫反应中发挥作用。此外,有机阴离子 5-氨基-4-咪唑甲酰胺核苷 (AICAR) 当磷酸化为 AICAR 单磷酸盐时,可作为 SLC19A1 逆向转运蛋白活性的底物。

种属

Human

表达系统

Sf9 insect cells

标签

N-Strep;C-Flag;N-8*His

蛋白编号

P41440 (V2-Q591)

基因 ID

6573

蛋白结构
N-term
8*His-Strep
SLC19A1 (V2-Q591)
Accession # P41440
Flag
C-term
同用名
SLC19A1; Reduced folate transporter; FOLT; Intestinal folate carrier 1; IFC-1; Placental folate transporter; Reduced folate carrier protein; RFC; hRFC; Reduced folate transporter 1; RFT-1; Solute carrier family 19 member 1; hSLC19A1
纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

SLC19A1 Protein, Human (Sf9, His, Strep, FLAG) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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  • 重组蛋白稀释计算器

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
SLC19A1 Protein, Human (Sf9, His, Strep, FLAG)
目录号:
HY-P702007
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