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  3. SLC29A2 蛋白, Human (Sf9, His, MBP, FLAG)

SLC29A2 蛋白, Human (Sf9, His, MBP, FLAG)

目录号: HY-P702030
产品使用指南

SLC29A2 蛋白是一种双向单向转运蛋白,可维持细胞核苷稳态。作为一种不依赖于 Na(+) 的转运蛋白,它促进各种核苷和核碱基的转运,包括肌苷、腺苷、尿苷、胸苷、胞苷和鸟苷。SLC29A2 蛋白, Human (Sf9, His, MBP, FLAG) 是重组的 SLC29A2 蛋白,由 Sf9 insect cells 表达,带有 N-MBP, C-Flag, N-8*His 标签。SLC29A2 蛋白, Human (Sf9, His, MBP, FLAG) 全长 455 个氨基酸。

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描述

The SLC29A2 protein is a bidirectional uniporter that maintains cellular nucleoside homeostasis. As a Na(+)-independent transporter, it facilitates the transport of various nucleosides and nucleobases, including inosine, adenosine, uridine, thymidine, cytidine, and guanosine. SLC29A2 Protein, Human (Sf9, His, MBP, FLAG) is the recombinant human-derived SLC29A2 protein, expressed by Sf9 insect cells , with N-MBP, C-Flag, N-8*His labeled tag. The total length of SLC29A2 Protein, Human (Sf9, His, MBP, FLAG) is 455 a.a., .

研究背景

双向单向转运蛋白 SLC29A2 蛋白在促进核苷和核碱基运输方面发挥着至关重要的作用,有助于维持细胞稳态。SLC29A2 作为 Na(+) 独立的被动转运蛋白,参与多种核苷的转运,包括肌苷、腺苷、尿苷、胸苷、胞苷和鸟苷,以及嘌呤核碱基(次黄嘌呤、腺嘌呤、鸟嘌呤)和嘧啶核碱基(胸腺嘧啶、尿嘧啶)。该蛋白参与肾细胞基底外侧膜的核苷转运,促进核苷代谢物的肝脏吸收。此外,SLC29A2 介导顶端核苷摄取到支持细胞中,调节睾丸中核苷跨血睾屏障的转运。在骨骼肌微血管内皮细胞中,它协调次黄嘌呤的流入和流出,从而控制可用于黄嘌呤氧化酶介导的活性氧(ROS)产生的细胞内次黄嘌呤水平。值得注意的是,SLC29A2 不充当腺苷或胸苷转运的核苷转运蛋白,并且不在细胞膜上表达。

种属

Human

表达系统

Sf9 insect cells

标签

N-MBP;C-Flag;N-8*His

蛋白编号

Q14542 (A2-L456)

基因 ID

3177

蛋白结构
N-term
8*His-MBP
SLC29A2 (A2-L456)
Accession # Q14542
Flag
C-term
同用名
SLC29A2; Equilibrative nucleoside transporter 2; 36 kDa nucleolar protein HNP36; Delayed-early response protein 12; Equilibrative nitrobenzylmercaptopurine riboside-insensitive nucleoside transporter; Equilibrative NBMPR-insensitive nucleoside transporter; Hydrophobic nucleolar protein; 36 kDa; Nucleoside transporter; ei-type; Solute carrier family 29 member 2
纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

SLC29A2 Protein, Human (Sf9, His, MBP, FLAG) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
SLC29A2 Protein, Human (Sf9, His, MBP, FLAG)
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HY-P702030
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