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  3. SLC35A1 蛋白, Human (Sf9, His, MBP, FLAG)

SLC35A1 蛋白, Human (Sf9, His, MBP, FLAG)

目录号: HY-P702008
产品使用指南

SLC35A1 是细胞内运输的关键参与者,充当将 CMP-唾液酸从细胞质运输到高尔基体的转运蛋白。作为反向转运蛋白,它将 CMP-唾液酸交换为 CMP,维持细胞稳态。SLC35A1 蛋白, Human (Sf9, His, MBP, FLAG) 是重组的 SLC35A1 蛋白,由 Sf9 insect cells 表达,带有 N-MBP, C-Flag, N-8*His 标签。SLC35A1 蛋白, Human (Sf9, His, MBP, FLAG) 全长 336 个氨基酸。

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描述

SLC35A1 is a key player in intracellular transport, acting as a transporter to transport CMP-sialic acid from the cytoplasm to the Golgi apparatus. As an antiporter, it exchanges CMP-sialic acid for CMP, maintaining cellular homeostasis. SLC35A1 Protein, Human (Sf9, His, MBP, FLAG) is the recombinant human-derived SLC35A1 protein, expressed by Sf9 insect cells , with N-MBP, C-Flag, N-8*His labeled tag. The total length of SLC35A1 Protein, Human (Sf9, His, MBP, FLAG) is 336 a.a., .

研究背景

SLC35A1 是细胞内转运过程中的关键蛋白,充当转运蛋白,促进 CMP-唾液酸从细胞质易位到高尔基体。作为反向转运蛋白,SLC35A1 参与 CMP-唾液酸与 CMP 的交换,展示了其在维持细胞稳态中的动态作用。虽然 SLC35A1 结合 CMP 唾液酸和游离 CMP,但它对游离 CMP 表现出更高的亲和力(通过相似性)。值得注意的是,这种多功能转运蛋白还可以将 CMP-唾液酸交换为 AMP 和 UMP,从而拓宽了其功能库。此外,SLC35A1 通过介导 CDP-核糖醇的转运(通过相似性)证明其参与细胞过程。

种属

Human

表达系统

Sf9 insect cells

标签

N-MBP;C-Flag;N-8*His

蛋白编号

P78382 (A2-V337)

基因 ID

10559

蛋白结构
N-term
8*His-MBP
SLC35A1 (A2-V337)
Accession # P78382
Flag
C-term
同用名
SLC35A1; CMP-sialic acid transporter; CMP-SA-Tr; CMP-Sia-Tr; Solute carrier family 35 member A1
纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

SLC35A1 Protein, Human (Sf9, His, MBP, FLAG) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
SLC35A1 Protein, Human (Sf9, His, MBP, FLAG)
目录号:
HY-P702008
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