SLC7A11 蛋白, Human (Cell-Free, His)

The SLC7A11 protein forms a heterodimer with SLC3A2 and acts as an antiporter to exchange extracellular L-cystine for intracellular L-glutamate across the plasma membrane. This sodium-independent electroneutral transport, with a 1:1 stoichiometry, relies on high intracellular levels of L-glutamate and intracellular reduction of L-cystine. SLC7A11 Protein, Human (Cell-Free, His) is the recombinant human-derived SLC7A11 protein, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of SLC7A11 Protein, Human (Cell-Free, His) is 501 a.a., with molecular weight of 58.2 kDa.

SLC7A11 蛋白与 SLC3A2 形成异二聚体，充当反向转运蛋白，促进细胞外阴离子 L-胱氨酸穿过细胞质膜交换为细胞内 L-谷氨酸。这种化学计量比为 1:1 的不依赖于钠的电中性转运是由细胞内高浓度的 L-谷氨酸和细胞内 L-胱氨酸的减少所推动的。SLC7A11 的关键作用延伸到提供 L-胱氨酸，以维持细胞外 L-胱氨酸和 L-半胱氨酸之间的氧化还原平衡，这对于细胞保护免受氧化应激至关重要。此外，它还介导 L-犬尿氨酸的输入，有助于抗铁死亡信号传导，这对于 L-胱氨酸和谷胱甘肽稳态至关重要。此外，SLC7A11 促进胎盘摄取 N-乙酰基-L-半胱氨酸，从而下调氧化应激、炎症和细胞凋亡相关途径。在体外，该蛋白质表现出转运 L-天冬氨酸的能力。除了其运输功能外，SLC7A11 可能在发育过程中的星形胶质细胞和脑膜细胞增殖中发挥作用，通过促进谷胱甘肽合成和从非神经元细胞（如星形胶质细胞和脑膜细胞）向未成熟神经元的输送来提供神经保护。值得注意的是，它控制着褐黑色素的直接产生。

Measured by its binding ability in a functional ELISA. Immobilized Human SLC7A11 at 2 μg/mL can bind Anti-SLC7A11 antibody, the EC₅₀ is 1.964-2.793 ng/mL.

Human

E. coli Cell-free

N-10*His

Q9UPY5 (M1-L501)

23657

MVRKPVVSTISKGGYLQGNVNGRLPSLGNKEPPGQEKVQLKRKVTLLRGVSIIIGTIIGAGIFISPKGVLQNTGSVGMSLTIWTVCGVLSLFGALSYAELGTTIKKSGGHYTYILEVFGPLPAFVRVWVELLIIRPAATAVISLAFGRYILEPFFIQCEIPELAIKLITAVGITVVMVLNSMSVSWSARIQIFLTFCKLTAILIIIVPGVMQLIKGQTQNFKDAFSGRDSSITRLPLAFYYGMYAYAGWFYLNFVTEEVENPEKTIPLAICISMAIVTIGYVLTNVAYFTTINAEELLLSNAVAVTFSERLLGNFSLAVPIFVALSCFGSMNGGVFAVSRLFYVASREGHLPEILSMIHVRKHTPLPAVIVLHPLTMIMLFSGDLDSLLNFLSFARWLFIGLAVAGLIYLRYKCPDMHRPFKVPLFIPALFSFTCLFMVALSLYSDPFSTGIGFVITLTGVPAYYLFIIWDKKPRWFRIMSEKITRTLQIILEVVPEEDKL

58.2 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm sterile filtered 25 mM HEPES, 150 mM NaCl, 0.05% Brij-78, 6% Trehalose, pH 7.4

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add 5-50% of glycerol (final concentration). Our default final concentration of glycerol is 50%. Customers could use it as reference.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.