SMAC/Diablo 蛋白, Human (His)

SMAC/Diablo protein is a core participant in apoptosis and activates caspases in the cytochrome c/Apaf-1/caspase-9 pathway. It antagonizes inhibitors of apoptosis proteins (IAPs), inhibits the binding of BIRC6/bruce to caspases and destabilizes XIAP/BIRC4. SMAC/Diablo Protein, Human (His) is the recombinant human-derived SMAC/Diablo protein, expressed by E. coli , with C-His labeled tag.

SMAC/Diablo 蛋白通过激活细胞色素 c/Apaf-1/caspase-9 通路中的 caspase，在细胞凋亡中发挥关键作用。其作用方式涉及抵消凋亡蛋白抑制剂（IAP）的抑制作用。SMAC/Diablo 通过阻止 BIRC6/bruce 与半胱天冬酶的结合，有效地阻碍 BIRC6/bruce 的活性。此外，它通过降低 XIAP/BIRC4 的稳定性和细胞凋亡抑制能力来发挥其调节影响。这是通过泛素-蛋白酶体途径促进 XIAP/BIRC4 泛素化和随后的降解来实现的。值得注意的是，SMAC/Diablo 破坏了 XIAP/BIRC4 和经过加工的 caspase-9 之间的相互作用，从而促进 caspase-3 的激活。从本质上讲，SMAC/Diablo 是控制细胞凋亡过程的复杂分子机制中的关键协调者。

Measured by its binding ability in a functional ELISA. Immobilized Human SMAC at 10 μg/mL (100 μL/well) can bind Biotinylated XIAP. The ED₅₀ for this effect is 0.328 μg/mL.

Human

E. coli

C-His

Q9NR28-1 (A56-D239)

56616  [NCBI]

AVPIAQKSEPHSLSSEALMRRAVSLVTDSTSTFLSQTTYALIEAITEYTKAVYTLTSLYRQYTSLLGKMNSEEEDEVWQVIIGARAEMTSKHQEYLKLETTWMTAVGLSEMAAEAAYQTGADQASITARNHIQLVKLQVEEVHQLSRKAETKLAEAQIEELRQKTQEEGEERAESEQEAYLRED

Approximately 22 kDa

Greater than 90% as determined by reducing SDS-PAGE

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 50 mM Tris-HCL, 300 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.