SNCA 蛋白, Human (His)

Alpha-synuclein (SNCA) is a key neuronal protein that regulates synaptic activity, including vesicle transport and neurotransmitter release. As a monomer, it enhances vesicle exocytosis, promotes fusion and fusion pore expansion, and increases local Ca(2+) release. SNCA Protein, Human (His) is the recombinant human-derived SNCA protein, expressed by E. coli , with N-6*His labeled tag. The total length of SNCA Protein, Human (His) is 140 a.a., with molecular weight of ~18.0 kDa.

α-突触核蛋白 (SNCA) 是一种关键神经元蛋白，在突触活动中协调多种作用，包括调节突触小泡运输和神经递质释放。作为单体，它积极参与突触小泡胞吐作用，增强囊泡启动、融合和胞吐融合孔的扩张，并从机制上增加微区的局部 Ca(2+) 释放，这对于 ATP 诱导的胞吐作用至关重要。在其多聚体膜结合状态下，SNCA 作为分子伴侣发挥作用，协助突触前质膜上的突触融合成分 (SNARE) 折叠，这一过程对于在衰老过程中维持正常的 SNARE 复合体组装至关重要。SNCA 还通过与多巴胺转运蛋白 (DAT1) 相互作用并调节其活性，在调节多巴胺神经传递中发挥着至关重要的作用。以可溶性单体和同四聚体的形式存在，四聚体和单体的动态细胞内群体共存，其中四聚体在维持体内平衡中发挥着重要作用。SNCA 参与与 UCHL1、synphilin-1/SNCAIP、CALM1、STXBP1、VAMP2、SNAP25、RPH3A、RAB3A、SERF1A 和 SEPTIN4 等蛋白质相互作用的复杂网络，强调其参与控制突触功能和完整性的复杂分子过程。

Immobilized SNCA at 1 μg/mL (100 μL/well) can bind SNCA Antibody , The ED₅₀ for this effect is 6.552-7.551 ng/mL.

Human

E. coli

N-6*His

P37840-1 (M1-A140)

6622  [NCBI]

MDVFMKGLSKAKEGVVAAAEKTKQGVAEAAGKTKEGVLYVGSKTKEGVVHGVATVAEKTKEQVTNVGGAVVTGVTAVAQKTVEGAGSIAAATGFVKKDQLGKNEEGAPQEGILEDMPVDPDNEAYEMPSEEGYQDYEPEA

Approximately 18.0 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of sterile 20 mM PB, 150 mM NaCl, pH 7.4 or 50 mM Tris-HCL, 300 mM NaCl, pH 7.4, 5% trehalose, 5% mannitol and 0.01% Tween 80 or PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.