SORD 蛋白, Human (HEK293, His)

SORD Protein is an enzyme that in humans is encoded by the SORD gene. SPRD is a polyol dehydrogenase that catalyzes the reversible NAD+-dependent oxidation of various sugar alcohols. SPRD is a key enzyme in the polyol pathway that interconverts glucose and fructose via sorbitol, which constitutes an important alternate route for glucose metabolism. SORD Protein, Human (HEK293, His) is the recombinant human-derived SORD protein, expressed by HEK293 , with C-6*His labeled tag. The total length of SORD Protein, Human (HEK293, His) is 356 a.a., with molecular weight of 39 kDa.

山梨醇脱氢酶 (SPRD) 是人类中由 SORD 基因编码的一种酶。SPRD 是一种多元醇脱氢酶，可催化多种糖醇的可逆 NAD+ 依赖性氧化，主要以 D-山梨醇 (D-葡萄糖醇)、L-苏糖醇、木糖醇和核糖醇为底物有活性，分别产生 C2 氧化产物 D-果糖、L-赤藓酮糖、D-木酮糖和 D-核酮糖。SPRD是多元醇途径中的关键酶，通过山梨醇将葡萄糖和果糖相互转化，构成葡萄糖代谢的重要替代途径。多元醇途径被认为涉及糖尿病并发症的病因学，例如由高血糖引起的糖尿病神经病变和视网膜病变。SPRD 可能通过使用山梨醇作为精子活力的替代能源而在精子活力中发挥作用。SPRD 可能在仲醇代谢中具有更普遍的功能，因为它还催化 (2R,3R)-2,3-丁二醇的立体定向氧化^[1][2][3][4]。

Human

HEK293

C-6*His

AAH21085.1 (A2-P357)

6652  [NCBI]

AAAAKPNNLSLVVHGPGDLRLENYPIPEPGPNEVLLRMHSVGICGSDVHYWEYGRIGNFIVKKPMVLGHEASGTVEKVGSSVKHLKPGDRVAIEPGAPRENDEFCKMGRYNLSPSIFFCATPPDDGNLCRFYKHNAAFCYKLPDNVTFEEGALIEPLSVGIHACRRGGVTLGHKVLVCGAGPIGMVTLLVAKAMGAAQVVVTDLSATRLSKAKEIGADLVLQISKESPQEIARKVEGQLGCKPEVTIECTGAEASIQAGIYATRSGGTLVLVGLGSEMTTVPLLHAAIREVDIKGVFRYCNTWPVAISMLASKSVNVKPLVTHRFPLEKALEAFETFKKGLGLKIMLKCDPSDQNP

39 kDa

Greater than 95% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 0.2 M NaCl, 5 mM DTT, 20% Glycerol, pH 8.0.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.