SPARC 蛋白, Human

SPARC Protein, Human is a Ca²⁺-binding glycoprotein that is differentially associated with morphogenesis, remodeling, cellular migration, and proliferation.

SPARC（分泌蛋白，酸性且富含半胱氨酸）是具有相似结构域结构的分泌基质细胞蛋白家族的创始成员。SPARC 显示出特定的效果，但通常会抑制粘附、扩散和增殖，并促进胶原蛋白基质的形成。对于内皮细胞，SPARC 会破坏粘着斑并结合和隔离 PDGF 和 VEGF^[1][2]。

The ED₅₀ is <3.0 μg/mL as measured by its ability to inhibit the cell growth of Mv1Lu mink lung epithelial cells, corresponding to a specific activity of >333 units/mg.

Human

E. coli

Tag Free

P09486 (A18-I303)

6678  [NCBI]

APQQEALPDETEVVEETVAEVTEVSVGANPVQVEVGEFDDGAEETEEEVVAENPCQNHHCKHGKVCELDENNTPMCVCQDPTSCPAPIGEFEKVCSNDNKTFDSSCHFFATKCTLEGTKKGHKLHLDYIGPCKYIPPCLDSELTEFPLRMRDWLKNVLVTLYERDEDNNLLTEKQKLRVKKIHENEKRLEAGDHPVELLARDFEKNYNMYIFPVHWQFGQLDQHPIDGYLSHTELAPLRAPLIPMEHCTTRFFETCDLDNDKYIALDEWAGCFGIKQKDIDKDLVI

Approximately 36.1 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Sage H, et al. SPARC, a secreted protein associated with cellular proliferation, inhibits cell spreading in vitro and exhibits Ca+2-dependent binding to the extracellular matrix. J Cell Biol. 1989 Jul;109(1):341-56.  [Content Brief]

  [2]. Alford AI, et al. Matricellular proteins: Extracellular modulators of bone development, remodeling, and regeneration. Bone. 2006 Jun;38(6):749-57.  [Content Brief]