SRP54 蛋白, Human (sf9, His)

SRP54 is an important component of the signal recognition particle (SRP) complex that drives co-translational targeting of secreted proteins to the endoplasmic reticulum (ER). SRP54 cooperates with the SRP receptor (SR) component SRPRA to bind to signal sequences on emerging ribosomal proteins. SRP54 Protein, Human (sf9, His) is the recombinant human-derived SRP54 protein, expressed by Sf9 insect cells , with N-His labeled tag. The total length of SRP54 Protein, Human (sf9, His) is 504 a.a., with molecular weight of ~58 kDa.

SRP54 是信号识别颗粒 (SRP) 复合物的重要组成部分，有助于将分泌蛋白和膜蛋白共翻译靶向内质网 (ER)。在 SRP 复合物中，SRP54 与 SRP 受体 (SR) 成分 SRPRA 结合，促进分泌蛋白靶向 ER 膜。SRP54 在此过程的最前沿发挥作用，当分泌前蛋白从核糖体中出现时，它会与它们的信号序列结合。值得注意的是，SRP54 表现出基础 GTP 酶活性，并在 SR 亚基 SRPRA 的相互 GTP 酶激活中协同作用，形成 GTP 依赖性复合物，这对于 SRP 介导的共翻译蛋白易位至 ER 至关重要。SRP9/SRP14 和/或 SRP19 的存在是 SRP54 与 RNA 稳定相互作用所必需的。除了在蛋白质易位中的作用之外，SRP54 在多种细胞过程中也发挥着重要作用，包括粒细胞增殖的调节、中性粒细胞迁移能力和外分泌胰腺的发育。

Human

Sf9 insect cells

N-His

P61011 (M1-M504)

6729  [NCBI]

Approximately 58 kDa

Greater than 95% as determined by reducing SDS-PAGE.

溶液

Supplied as a 0.2 μm filtered solution of 20 mM Tris, pH 7.5, 300 mM NaCl, 10% Glycerol.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.