ssPA 蛋白, E. coli (His)

The ssPA protein specifically forms an equimolar complex with RNA polymerase holoenzyme (RNAP), thereby distinguishing its interaction preference from the core enzyme. ssPA is primarily synthesized during amino acid starvation and accounts for more than 50% of the total protein produced under these conditions. ssPA Protein, E. coli (His) is the recombinant E. coli-derived ssPA protein, expressed by E. coli , with N-His labeled tag. The total length of ssPA Protein, E. coli (His) is 212 a.a., with molecular weight of ~25 kDa.

ssPA（严格饥饿蛋白 A）与 RNA 聚合酶全酶 (RNAP) 形成等摩尔复合物，但不与核心酶结合。ssPA 主要在氨基酸饥饿期间合成，占此类条件下合成的总蛋白质的 50% 以上。它的作用对于协调从 P1 早期到 P1 晚期基因表达的转变至关重要，表明它参与调节特定细胞状态下的基因表达。值得注意的是，Rnk 和 SspA 与铜绿假单胞菌藻酸盐调节基因 algR2 之间观察到的功能互换性强调了 ssPA 样蛋白在细菌系统中的调节多功能性。

E.coli

E. coli

N-His

P0ACA3 (M1-S212)

61751941

MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKDNPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLYMHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCYLAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS

Approximately 25 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.