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  3. ssPA 蛋白, E. coli (His)

ssPA 蛋白, E. coli (His)

目录号: HY-P76089
COA 产品使用指南

ssPA 蛋白与 RNA 聚合酶全酶 (RNAP) 特异性形成等摩尔复合物,从而将其相互作用偏好与核心酶区分开来。ssPA 主要在氨基酸饥饿期间合成,占这些条件下产生的总蛋白质的 50% 以上。ssPA 蛋白, E. coli (His) 是重组的 ssPA 蛋白,由 E. coli 表达,带有 N-His 标签。ssPA 蛋白, E. coli (His) 全长 212 个氨基酸,分子量约为 ~25 kDa。

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10 μg ¥1070 In-stock
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描述

The ssPA protein specifically forms an equimolar complex with RNA polymerase holoenzyme (RNAP), thereby distinguishing its interaction preference from the core enzyme. ssPA is primarily synthesized during amino acid starvation and accounts for more than 50% of the total protein produced under these conditions. ssPA Protein, E. coli (His) is the recombinant E. coli-derived ssPA protein, expressed by E. coli , with N-His labeled tag. The total length of ssPA Protein, E. coli (His) is 212 a.a., with molecular weight of ~25 kDa.

研究背景

ssPA(严格饥饿蛋白 A)与 RNA 聚合酶全酶 (RNAP) 形成等摩尔复合物,但不与核心酶结合。ssPA 主要在氨基酸饥饿期间合成,占此类条件下合成的总蛋白质的 50% 以上。它的作用对于协调从 P1 早期到 P1 晚期基因表达的转变至关重要,表明它参与调节特定细胞状态下的基因表达。值得注意的是,Rnk 和 SspA 与铜绿假单胞菌藻酸盐调节基因 algR2 之间观察到的功能互换性强调了 ssPA 样蛋白在细菌系统中的调节多功能性。

种属

E.coli

表达系统

E. coli

标签

N-His

蛋白编号

P0ACA3 (M1-S212)

基因 ID

61751941

蛋白结构
N-term
His
ssPA (M1-S212)
Accession # P0ACA3
C-term
同用名
Stringent starvation protein A; sspA; pog; ssp
氨基酸序列

MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKDNPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLYMHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCYLAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS

分子量

Approximately 25 kDa

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

ssPA Protein, E. coli (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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ssPA Protein, E. coli (His)
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HY-P76089
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