1. 重组蛋白
  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. ST6GALNAC2 蛋白, Human (HEK293, His)

ST6GALNAC2 蛋白, Human (HEK293, His)

目录号: HY-P71332
COA 产品使用指南

ST6GALNAC2 蛋白是一种必需酶,可催化 N-乙酰神经酰胺基团转移至糖蛋白中的聚糖链。该糖基转移酶显示出对 N-乙酰半乳糖胺 (GalNAc) 残基的偏好,这些残基已通过添加半乳糖或半乳糖然后在 α-2,3 连接中添加唾液酸进行修饰。ST6GALNAC2 蛋白, Human (HEK293, His) 是重组的 ST6GALNAC2 蛋白,由 HEK293 表达,带有 C-6*His 标签。ST6GALNAC2 蛋白, Human (HEK293, His) 全长 346 个氨基酸,分子量约为 ~44.0 kDa。

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规格 价格 是否有货 数量
10 μg ¥1400 In-stock
50 μg ¥4200 In-stock
100 μg   询价  

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描述

The ST6GALNAC2 protein is an essential enzyme that catalyzes the transfer of N-acetylneuramidyl groups to glycan chains in glycoproteins. This glycosyltransferase shows a preference for N-acetylgalactosamine (GalNAc) residues that have been modified by the addition of galactose or galactose followed by the addition of sialic acid in an α-2,3 linkage. ST6GALNAC2 Protein, Human (HEK293, His) is the recombinant human-derived ST6GALNAC2 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of ST6GALNAC2 Protein, Human (HEK293, His) is 346 a.a., with molecular weight of ~44.0 kDa.

研究背景

ST6 N-乙酰半乳糖胺 Alpha-2,6-唾液酸转移酶 2 (ST6GALNAC2) 是一种催化 N-乙酰神经氨酰基转移到糖蛋白聚糖链上的酶。这种唾液酸转移酶表现出对聚糖结构的偏好,其中 N-乙酰半乳糖胺 (GalNAc) 残基已经通过添加半乳糖或半乳糖,然后添加 α-2,3 连接唾液酸进行修饰。ST6GALNAC2 的酶活性有助于将唾液酸残基添加到糖蛋白上,从而调节其功能特性和相互作用。唾液酸化是一种重要的翻译后修饰,对细胞粘附、免疫反应和信号传导具有影响。了解 ST6GALNAC2 的底物特异性可以深入了解其在聚糖结构复杂调节中的作用及其对不同细胞过程的影响。

生物活性

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

种属

Human

表达系统

HEK293

标签

C-6*His

蛋白编号

Q9UJ37 (S29-R374)

基因 ID

10610  [NCBI]

蛋白结构
N-term
ST6GALNAC2 (S29-R374)
Accession # Q9UJ37
6*His
C-term
同用名
Alpha-N-Acetylgalactosaminide Alpha-2; 6-Sialyltransferase 2; GalNAc Alpha-2; 6-Sialyltransferase II; ST6GalNAc II; ST6GalNAcII; SThM; Sialyltransferase 7B; SIAT7-B; ST6GALNAC2; SIAT7B; SIATL1; STHM
氨基酸序列

SAVQRYPGPAAGARDTTSFEAFFQSKASNSWTGKGQACRHLLHLAIQRHPHFRGLFNLSIPVLLWGDLFTPALWDRLSQHKAPYGWRGLSHQVIASTLSLLNGSESAKLFAPPRDTPPKCIRCAVVGNGGILNGSRQGPNIDAHDYVFRLNGAVIKGFERDVGTKTSFYGFTVNTMKNSLVSYWNLGFTSVPQGQDLQYIFIPSDIRDYVMLRSAILGVPVPEGLDKGDRPHAYFGPEASASKFKLLHPDFISYLTERFLKSKLINTHFGDLYMPSTGALMLLTALHTCDQVSAYGFITSNYWKFSDHYFERKMKPLIFYANHDLSLEAALWRDLHKAGILQLYQR

分子量

Approximately 44.0 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

溶液.

组分

Supplied as a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, 5 mM EDTA, 5% Trehalosen, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

保存条件 & 期限

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

运输条件

Shipping with dry ice.

文件资料

ST6GALNAC2 Protein, Human (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
ST6GALNAC2 Protein, Human (HEK293, His)
目录号:
HY-P71332
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