Staphopain B 蛋白, S. aureus (GST)

Staphopain B is a cysteine protease that severely disrupts host immunity by degrading elastin, fibrin, fibronectin, and kininogen. It blocks phagocytosis of opsonized Staphylococcus aureus and induces neutrophil and monocyte death through proteolytic activity. Staphopain B Protein, S. aureus (GST) is the recombinant Staphylococcus aureus-derived Staphopain B protein, expressed by E. coli , with N-GST labeled tag.

Staphopain B 是一种半胱氨酸蛋白酶，通过靶向多种宿主蛋白，在破坏宿主先天免疫反应中发挥着关键作用。它具有降解宿主弹性蛋白、纤维原、纤连蛋白和激肽原的能力。此外，Stahopain B 通过以蛋白水解活性依赖性方式诱导中性粒细胞和单核细胞死亡，阻碍调理后的金黄色葡萄球菌的吞噬作用，从而干扰宿主的防御机制。这种抑制作用延伸至中性粒细胞上“别吃我”信号 CD31 的下调。此外，Stahopain B 会裂解宿主 galectin-3/LGALS3，从而阻碍该凝集素的中性粒细胞激活能力。值得注意的是，葡萄球菌蛋白酶 B 的过早激活/折叠可以通过葡萄球菌抑素 B (SspC) 进行调节，这可能通过防止 SspB 降解葡萄球菌细胞质蛋白来发挥保护作用。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Staphylococcus aureus

E. coli

N-GST

Q99V46 (D220-Y393)

/

DQVQYENTLKNFKIREQQFDNSWCAGFSMAALLNATKNTDTYNAHDIMRTLYPEVSEQDLPNCATFPNQMIEYGKSQGRDIHYQEGVPSYNQVDQLTKDNVGIMILAQSVSQNPNDPHLGHALAVVGNAKINDQEKLIYWNPWDTELSIQDADSSLLHLSFNRDYNWYGSMIGY

Approximately 46.9 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against solution in 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.