Stratifin 蛋白, Human (N-His, C-Myc)

Stratifin (14-3-3 sigma), encoded by SFN, functions as a multifaceted adapter protein that binds to partners through phosphoserine or phosphothreonine motifs and participates in a variety of cellular processes. It regulates epithelial cell growth and protein synthesis through keratin 17 (KRT17), and may affect MDM2 autoubiquitination and activate p53. Stratifin Protein, Human (N-His, C-Myc) is the recombinant human-derived Stratifin protein, expressed by E. coli , with N-10*His, C-Myc labeled tag.

Stratifin，也称为14-3-3 sigma，由SFN基因编码，是属于14-3-3家族的多功能蛋白。它作为衔接蛋白，通过识别磷酸丝氨酸或磷酸苏氨酸基序与众多伙伴结合，参与多种细胞过程。其关键作用包括与角蛋白 17 (KRT17) 结合时调节上皮细胞生长和蛋白质合成，以及潜在参与 MDM2 自动泛素化和降解，从而导致肿瘤抑制因子 p53 的激活。stratifin 以同二聚体形式存在并参与各种蛋白质复合物，可与多种蛋白质相互作用，例如 GAB2、SAMSN1、SRPK2、COPS6、COP1、DAPK2、PI4KB、SLITRK1 和 LRRK2，展示了其在协调关键细胞过程和信号通路。

Measured by its ability to inhibit GBP1(HY-P75172) to catalyze 5mM GTP (HY-W010737) substrates that incubate at room temperature for 10 minutes. The specific activity is 281.83 nmol/min/mg.

Human

E. coli

N-10*His;C-Myc

P31947-1 (M1-S248)

2810  [NCBI]

MERASLIQKAKLAEQAERYEDMAAFMKGAVEKGEELSCEERNLLSVAYKNVVGGQRAAWRVLSSIEQKSNEEGSEEKGPEVREYREKVETELQGVCDTVLGLLDSHLIKEAGDAESRVFYLKMKGDYYRYLAEVATGDDKKRIIDSARSAYQEAMDISKKEMPPTNPIRLGLALNFSVFHYEIANSPEEAISLAKTTFDEAMADLHTLSEDSYKDSTLIMQLLRDNLTLWTADNAGEEGGEAPQEPQS

Approximately 34 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0 or PBS, pH 7.0, 10% trehalose, 0.02% Tween80.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.