SULT1E1 蛋白, Human (N-His)

The SULT1E1 protein is a sulfotransferase that critically regulates estrogen homeostasis by sulfating estradiol and estrone, resulting in hormone inactivation. Its versatility extends to sulfated DHEA, pregnenolone, and xenobiotics such as ethinyl estradiol. SULT1E1 Protein, Human (N-His) is the recombinant human-derived SULT1E1 protein, expressed by E. coli , with N-His labeled tag.

SULT1E1 蛋白是一种利用 3'-磷酸-5'-腺苷基硫酸盐 (PAPS) 作为磺酸盐供体的磺基转移酶，通过催化雌二醇和雌酮的硫酸盐结合，从而导致这些激素失活，从而在雌激素稳态中发挥关键作用。除了其在雌激素代谢中的主要功能外，SULT1E1 还通过硫酸化脱氢表雄酮 (DHEA)、孕烯醇酮、(24S)-羟基胆固醇和各种外源化合物（如炔雌醇、烯雌宁、二乙基己烯雌醇和 1-萘酚）展现出多功能性，尽管效率较低。值得注意的是，SULT1E1 不会磺化皮质醇、睾酮和多巴胺。此外，这种磺基转移酶可能与肠道微生物群-宿主代谢相互作用有关，因为它对 4-乙基苯酚 (4-EP) 进行 O-磺化，而 4-乙基苯酚 (4-EP) 是肠道细菌产生的酪氨酸衍生代谢物。由此产生的产物 4-EPS 可穿过血脑屏障，并可能对少突胶质细胞的成熟和髓鞘形成产生调节作用，从而影响与边缘系统相关的大脑区域的功能连接。SULT1E1 的多种底物特异性凸显了其在激素调节中的关键作用，并表明其参与更广泛的生理过程，包括对神经功能具有潜在影响的肠脑相互作用。

Measured by its ability to transfer sulfate from PAPS to 1-Napthol. The specific activity is 74.44 pmol/min/μg that incubate at 37 ºC for 20 minutes.

Human

E. coli

N-6*His

P49888 (N2-I294)

6783

NSELDYYEKFEEVHGILMYKDFVKYWDNVEAFQARPDDLVIATYPKSGTTWVSEIVYMIYKEGDVEKCKEDVIFNRIPFLECRKENLMNGVKQLDEMNSPRIVKTHLPPELLPASFWEKDCKIIYLCRNAKDVAVSFYYFFLMVAGHPNPGSFPEFVEKFMQGQVPYGSWYKHVKSWWEKGKSPRVLFLFYEDLKEDIRKEVIKLIHFLERKPSEELVDRIIHHTSFQEMKNNPSTNYTTLPDEIMNQKLSPFMRKGITGDWKNHFTVALNEKFDKHYEQQMKESTLKFRTEI

Approximately 36 kDa

Greater than 90% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of 50 mM Tris-HCL, 200 mM NaCl, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.