Syndecan-3 蛋白, Human (HEK293, His)

Syndecan-3 Protein, Human (HEK293, His) is a Dendritic cells (DCs)-specific heparan sulfate (HS) proteoglycan that captures HIV-1 through interaction with the HIV-1 envelope glycoprotein gp120. SDC3 is involved in the regulation of growth factor signalling, Notch and BMP signalling, adhesion and migration, proliferation and differentiation. Syndecan-3 is associated with the control of feeding behaviour and the generation of cerebellar fibrillar plaques in Alzheimer鈥檚 disease^[1][2][3]. Syndecan-3 Protein, Human (HEK293, His) is the recombinant human-derived Syndecan-3 protein, expressed by HEK293 , with C-His labeled tag. The total length of Syndecan-3 Protein, Human (HEK293, His) is 311 a.a., with molecular weight of 45-100 kDa.

多聚糖 (sdcs) 是硫酸乙酰肝素蛋白聚糖 (HSPG)，由共价连接有硫酸乙酰肝素 (HS) 糖胺聚糖链的核心蛋白组成。这些分子形成糖萼的一部分，包括内皮细胞表面的膜结合蛋白聚糖和糖蛋白网络。有四种哺乳动物多配体，称为多配体-1 (sdc-1)、-2、-3 和-4。Sdc-3 是神经系统中主要的多聚糖，它是在神经系统中首次被发现的，并且与阿尔茨海默病中进食行为的控制和小脑纤维斑块的生成有关。Sdc-3 也是肌肉骨骼系统的 HSPG^[1]。
Syndecan-3 可稳定捕获的病毒，增强顺式树突状细胞 (DC) 感染，并促进向 T 细胞的传播。通过肝素酶 III 从细胞表面去除 HS 或通过 siRNA 沉默 syndecan-3 可以部分抑制未成熟 DC 的 HIV-1 传播，而中和 syndecan-3 和 DC-SIGN 则完全消除 HIV-1 捕获和随后的传播。Syndecan-3 (SDC3) 具有所有四种哺乳动物 Syndecan 中最大的核心蛋白，并且可以同时含有硫酸乙酰肝素和硫酸软骨素链^[2][3]。

Human

HEK293

C-His

ABM82826 (M1-K311)

9672  [NCBI]

MAIAYLGSSCPSQPPSSLALSLSPTPSDFEQESGIETAMRFSPDVALAVSTTPFEELPSERPTLEPATSPLVVTEVPEEPSQRATTVSTTMATTAATSTGDPTVATVPATVATATPSTPAAPPFTATTAVIRTTGVRRLLPLPLTTVATARATTPEAPSPPTTAAVLDTEAPTPRLVSTATSRPRALPRPATTQEPDIPERSTLPLGTTAPGPTEVAQTPTPETFLTTIRDEPEVPVSGGPSGDFELPEEETTQPDTANEVVAVGGAAAKASSPPGTLPKGARPGPGLLDNAIDSGSSAAQLPQKSILERK

Approximately 100 kDa due to the glycosylation.

Greater than 85% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.