T-PA 蛋白, Human (HEK293, His)

T-PA is a key proteolytic enzyme that coordinates the conversion of the abundant but inactive zymogen plasminogen to its active form plasmin via hydrolysis of a single Arg-Val bond. This enzymatic conversion helps regulate plasmin-mediated proteolysis, a key process in tissue remodeling, degradation, and various pathophysiological events. T-PA Protein, Human (HEK293, His) is the recombinant human-derived T-PA protein, expressed by HEK293 , with C-6*His labeled tag.

组织纤溶酶原激活剂 (t-PA) 是一种关键蛋白，可催化丰富但无活性的酶原纤溶酶原转化为活性蛋白酶纤溶酶，这是通过水解纤溶酶原中的单个 Arg-Val 键来实现的。这种酶促过程是控制纤溶酶介导的蛋白水解过程中不可或缺的一部分，从而在组织重塑、降解和细胞迁移以及影响各种病理生理学事件中发挥着至关重要的作用。此外，t-PA 在卵母细胞激活过程中表现出功能意义，参与透明带反应中的皮质颗粒反应，有助于预防多精受精，并在受精过程中充当保护机制（通过相似性）。t-PA 的多方面作用强调了其在生理和生殖过程中的重要性，强调了其在蛋白水解和细胞事件中的调节功能。

Measured by its ability to cleave a peptide substrate, N-carbobenzyloxy-Gly-Gly-Arg-7-amido-4-methylcoumarin (Z-GGR-AMC). Read at excitation and emission wavelengths of 380 nm and 460 nm (top read). The specific activity is >500.47 pmol/min/µg, as measured under the described conditions.

Human

HEK293

C-6*His

P00750-1 (S36-P562)

5327  [NCBI]

SYQVICRDEKTQMIYQQHQSWLRPVLRSNRVEYCWCNSGRAQCHSVPVKSCSEPRCFNGGTCQQALYFSDFVCQCPEGFAGKCCEIDTRATCYEDQGISYRGTWSTAESGAECTNWNSSALAQKPYSGRRPDAIRLGLGNHNYCRNPDRDSKPWCYVFKAGKYSSEFCSTPACSEGNSDCYFGNGSAYRGTHSLTESGASCLPWNSMILIGKVYTAQNPSAQALGLGKHNYCRNPDGDAKPWCHVLKNRRLTWEYCDVPSCSTCGLRQYSQPQFRIKGGLFADIASHPWQAAIFAKHRRSPGERFLCGGILISSCWILSAAHCFQERFPPHHLTVILGRTYRVVPGEEEQKFEVEKYIVHKEFDDDTYDNDIALLQLKSDSSRCAQESSVVRTVCLPPADLQLPDWTECELSGYGKHEALSPFYSERLKEAHVRLYPSSRCTSQHLLNRTVTDNMLCAGDTRSGGPQANLHDACQGDSGGPLVCLNDGRMTLVGIISWGLGCGQKDVPGVYTKVTNYLDWIRDNMRP

Approximately 60-75 kDa due to the glycosylation

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM Succinate, 4% Trehalose, 4% Mannitol, 0.2 mM CaCl₂,0.02% Tween 80, pH 4.5 or 20 mM MES, 150 mM NaCl, pH 5.5 or 20 mM MES, 150 mM NaCl, pH 5.5.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.