TARDBP 蛋白, Human (P.pastoris, His)

TARDBP protein is an RNA-binding factor with RRM1 and RRM2 that binds GU repeats and regulates RNA splicing in long introns and 3'UTRs.It affects proteins critical for neuronal survival and neurodegenerative diseases.TARDBP Protein, Human (P.pastoris, His) is the recombinant human-derived TARDBP protein, expressed by P.pastoris , with N-6*His labeled tag.

TARDBP 蛋白是一种 RNA 结合因子，在 RNA 生物发生和加工的各个步骤中发挥多方面的作用。利用其两个 RNA 识别基序 RRM1 和 RRM2，TARDBP 优先结合 RNA 分子上的 GU 重复序列，主要位于长内含子和 mRNA 的 3'UTR 内。这种结合活性调节大量非编码和蛋白质编码 RNA 的剪接，影响对神经元存活和与神经退行性疾病相关的至关重要的蛋白质。TARDBP 还通过调节线粒体转录本的加工来促进线粒体稳态，并通过将 CNOT7/CAF1 去腺苷酶募集到 mRNA 3'UTR 来调节 mRNA 稳定性，从而导致聚 (A) 尾去腺苷化。为了应对氧化损伤，TARDBP 与应激颗粒中停滞的核糖体结合，有助于细胞存活。此外，它通过形成细胞质肌颗粒和结合编码肌节蛋白的 mRNA 参与骨骼肌的形成和再生。此外，TARDBP 通过以 FBXL3 依赖性方式稳定 CRY1 和 CRY2 蛋白来参与生物钟周期性的维持，并负向调节 CDK6 的表达。与多种蛋白质（包括 BRDT、ATXN2、MATR3、UBQLN2、HNRNPA2B1、ZNF106、CNOT7/CAF1、CRY2 和 PPIA/CYPA）的相互作用凸显了 TARDBP 在 RNA 调节和细胞过程中的多种作用。

Human

P. pastoris

N-6*His

Q13148 (M1-G396)

23435  [NCBI]

MSEYIRVTEDENDEPIEIPSEDDGTVLLSTVTAQFPGACGLRYRNPVSQCMRGVRLVEGILHAPDAGWGNLVYVVNYPKDNKRKMDETDASSAVKVKRAVQKTSDLIVLGLPWKTTEQDLKEYFSTFGEVLMVQVKKDLKTGHSKGFGFVRFTEYETQVKVMSQRHMIDGRWCDCKLPNSKQSQDEPLRSRKVFVGRCTEDMTEDELREFFSQYGDVMDVFIPKPFRAFAFVTFADDQIAQSLCGEDLIIKGISVHISNAEPKHNSNRQLERSGRFGGNPGGFGNQGGFGNSRGGGAGLGNNQGSNMGGGMNFGAFSINPAMMAAAQAALQSSWGMMGMLASQQNQSGPSGNNQNQGNMQREPNQAFGSGNNSYSGSNSGAAIGWGSASNAGSGSG

Approximately 44.9 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized after extensive dialysis against solution in 20 mM Tris-HC1, 0.5 M NaCl, 6% Trehalose, pH 8.0 or PBS, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.